肉桂和丁香中α-葡萄糖苷酶抑制剂的筛选  

Screening ofα-glucosidase inhibitors in Eugenia caryophyllata and Cinnamomum cassia

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作  者:徐聪 XU Cong(Ankang Municipality Agricultural Science Research Institute,Ankang725000,Shaanxi,China)

机构地区:[1]安康市农业科学研究院,陕西安康725000

出  处:《湖北农业科学》2025年第3期107-111,共5页Hubei Agricultural Sciences

基  金:陕西省创新能力支撑计划项目(2022PT-21)。

摘  要:为探究药食同源植物肉桂和丁香中具有α-葡萄糖苷酶抑制作用的潜在活性物质,采用体外酶活性模型实验测定肉桂和丁香甲醇粗提物对α-葡萄糖苷酶的抑制作用,进一步采用生物活性色谱和超高效液相色谱-串联四级杆飞行时间质谱(UPLC-QTOF-MS/MS)分析其中的活性物质。结果表明,肉桂和丁香甲醇粗提物具有较强的α-葡萄糖苷酶抑制作用,IC_(50)分别为(50.0±0.08)μg/mL和(2.19±0.17)μg/mL,抑制作用明显高于阳性对照阿卡波糖[IC_(50)=(264.37±2.73)μg/mL]。生物活性色谱分析表明多酚类物质为主要活性成分。UPLC-QTOF-MS/MS分析揭示丁香中的黄酮类物质、肉桂中的肉桂酸和2-甲氧基肉桂醛可能为潜在的活性成分。肉桂和丁香在预防和治疗糖尿病的功能性利用方面具有一定潜力。The purpose of this study was to explore the potential constituents responsible forα-glucosidase inhibitory activity of medicine food homologous herbs Eugenia caryophyllata and Cinnamomum cassia.Theα-glucosidase inhibitory effect of crude extracts from Eugenia caryophyllata and Cinnamomum cassia were preliminary determined by in vitro enzyme activity experiment.The corresponding bioactive constituents were determined by the combined use of high-resolution inhibition profiling and ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS/MS).The results showed that,crude methanol extracts of Eugenia caryophyllata and Cinnamomum cassia displayed considerable inhibition towardsα-glucosidase with IC50 values of(50.0±0.08)μg/mL and(2.19±0.17)μg/mL,respectively,more active than the positive control acarbose[IC50=(264.37±2.73)μg/mL].The high-resolution inhibition profiles pointed out that polyphenols were correlated withα-glucosidase inhibitory activity.In addition,flavonoids from Eugenia caryophyllata,cinnamic acid and 2′-methoxy cinnamaldehyde from Cinnamomum cassia were considered as the possible inhibitors by the careful analysis of UPLC-QTOF-MS/MS data.The results supported further research with the aim of developing Eugenia caryophyllata and Cinnamomum cassia into functional food for prevention and management of T2D and T2D-related complications.

关 键 词:药食同源植物 Α-葡萄糖苷酶 生物活性色谱 超高效液相色谱-串联四级杆飞行时间质谱(UPLC-QTOF-MS/MS) 

分 类 号:R284.1[医药卫生—中药学]

 

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