转录组和代谢组联合分析挖掘灰毡毛忍冬苯丙素类物质生物合成途径关键基因  

Key Genes in Phenylpropanoid Biosynthesis Pathway of Lonicera macranthoides Based on Transcriptome and Metabolome Conjoint Analysis

作　　者：何佳蔚 张惊宇 曾娟 朱佳源 周思敏 瞿美玲 周日宝[1,2,3,4] 刘湘丹 HE Jiawei;ZHANG Jingyu;ZENG Juan;ZHU Jiayuan;ZHOU Simin;QU Meiling;ZHOU Ribao;LIU Xiangdan(School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;Key Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale GenuineMedicinal Materials,Changsha 410208,China;Key Laboratory of Traditional Chinese Medicine Modernization Research in General Colleges andUniversities of Hunan Province,Changsha 410208,China;Hunan Traditional Chinese Medicine Piece Standardization and Function Technology Research Center,Changsha 410208,China)

机构地区：[1]湖南中医药大学药学院,长沙410208 [2]湘产大宗道地药材种质资源及规范化种植重点研究室,长沙410208 [3]湖南省普通高等学校中药现代化研究重点实验室,长沙410208 [4]湖南省中药饮片标准化及功能工程技术研究中心,长沙410208

出　　处：《中国实验方剂学杂志》2025年第8期167-175,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(82373992);国家现代农业产业技术体系(CARS-21);湖南省中药材产业技术体系专项(HARS-11);湖南省自然科学基金项目(2023JJ60477);2020年湖南省一流专业建设点:中药资源与开发;湖南中医药大学重点学科中药学科(校行发规字【2023】2号);湖南中医药大学研究生创新课题项目(2024CX094);湖南中医药大学校级科研基金项目(2022YYZK003);湖南中医药大学本科生科研创新基金项目(2023BKS102);湖南省研究生科研创新项目(CX20230803)。

摘　　要：目的:基于转录组和代谢组联合分析挖掘灰毡毛忍冬中苯丙素类化合物生物合成途径关键基因,为进一步探究湘蕾型灰毡毛忍冬中苯丙素类化合物的合成调控机制提供基础。方法:选取湘蕾型灰毡毛忍冬茎、叶和3个花期花作为实验材料构建转录组和代谢组,通过京都基因与基因组百科全书(KEGG)数据库、加权共表达网络分析(WGCNA)对转录组和代谢组进行联合分析,挖掘灰毡毛忍冬苯丙素类物质生物合成途径关键基因。结果:该研究发现77个差异苯丙素类化合物和315个差异基因,通过转录代谢联合分析,最终挖掘出9个关键的差异代谢物和4个与其相关的关键基因,其中肉桂酸、5-O-咖啡酰莽草酸、芥子醇和绿原酸在花中较高,其中标志性有效成分绿原酸在枯萎期中含量急剧下降,咖啡酸、阿魏酸、5-羟基松柏醛、对羟基肉桂醇和紫丁香苷在叶中含量较高,这4个关键基因分别属于肉桂醇脱氢酶(CAD)家族、4-香豆酸:辅酶A(4CL)家族、羟基肉桂酰基转移酶(HCT)家族和L-苯丙氨酸解氨酶(PAL)家族基因。结论:灰毡毛忍冬中挖掘出的4个关键基因中TRINITY_DN42767_c0_g6与对羟基肉桂醇、芥子醇的合成相关,TRINITY_DN43525_c4_g1以咖啡酸、阿魏酸、肉桂酸为底物催化下一步反应,TRINITY_DN47958_c3_g4与3-对香豆酰基奎宁酸和咖啡酰辅酶A的合成相关,TRINITY_DN52595_c1_g2与肉桂酸的合成相关。该发现为进一步探究湘蕾型灰毡毛忍冬中苯丙素类化合物的合成调控机制提供基础。Objective:Based on the conjoint analysis of transcriptome and metabolome,the key genes in the phenylpropanoid biosynthesis pathway of Lonicera macranthoides were explored,which provided a basis for further exploring the synthesis and regulation mechanism of phenylpropanoid compounds in"Xianglei"L.macranthoides.Methods:The stem,leaves,and three flowering flowers of"Xianglei"L.macranthoides were selected as experimental materials to construct transcriptome and metabolome.The transcriptome and metabolomics were conjointly analyzed by the Kyoto Encyclopedia of Genes and Genomes(KEGG)database and weighted correlation network analysis(WGCNA),and the key genes in the phenylpropanoid biosynthesis pathway of L.macranthoides were explored.Results:In this study,77 differential phenylpropanoids and 315 differential genes were found.Through the joint analysis of transcription and metabolism,nine key differential metabolites and four key genes related to them were finally discovered.Among them,cinnamic acid,5-O-caffeoylshikimic acid,sinapyl alcohol,and chlorogenic acid were higher in flowers,and the content of the iconic effective component,namely chlorogenic acid,decreased sharply during the withering period.Caffeic acid,ferulic acid,5-hydroxyconiferaldehyde,p-coumaryl alcohol,and syringin were higher in leaves.These four key genes belong to the cinnamic alcohol dehydrogenase(CAD)family,4-coumaric acid:Coenzyme A(4CL)family,hydroxycinnamyl transferase(HCT)family,and L-phenylalanine ammonlyase(PAL)family genes.Conclusion:Among the four key genes excavated from L.macranthoides,TRINITY_DN42767_c0_g6 is related to the synthesis of p-coumaryl alcohol and sinapyl alcohol.TRINITY_DN43525_c4_g1 uses caffeic acid,ferulic acid,and cinnamic acid as substrates to catalyze the next reaction.TRINITY_DN47958_c3_g4 correlates with the synthesis of 3-p-coumaroyl quinic acid and caffeoyl-CoA,and TRINITY_DN52595_c1_g2 correlates with cinnamic acid synthesis.These findings provide a basis for further exploring the synthesis and regulation mechan

关 键 词：灰毡毛忍冬 苯丙素类化合物 转录组 代谢组 联合分析 关键基因 

分 类 号：R284[医药卫生—中药学] S567.79[医药卫生—中医学] Q943.2[农业科学—中草药栽培]