儿童重度抑郁症患者肠道菌群及其系统性免疫应答研究  

Gut dysbiosis and systemic inflammation among children with major depressive disorder

作　　者：赖韵如 程译文 朱张骋 赵龙友 宋清海 LAI Yunru;CHENG Yiwen;ZHU Zhangcheng;ZHAO Longyou;SONG Qinghai(Department of Psychiatry,Lishui Second People's Hospital,Lishui,Zhejiang 323000,China;不详)

机构地区：[1]丽水市第二人民医院精神科,浙江323000 [2]浙江大学医学院附属第一医院传染病重症诊治全国重点实验室 [3]温州医科大学预防医学系

出　　处：《中国微生态学杂志》2025年第2期215-221,共7页Chinese Journal of Microecology

基　　金：浙江省自然科学基金(LQ24H090005)。

摘　　要：目的探讨重度抑郁症(MDD)患儿肠道菌群变化规律及其系统性炎症变化。方法本研究招募了39名新诊断的MDD患儿及39名健康儿童为研究对象。采集全部对象粪便和血清样本,并提取细菌基因组DNA进行MiSeq高通量测序,利用Bio-Plex技术分析血清中的炎症因子,比较组间菌群结构和组成差异及患儿炎症反应的差异。结果MDD患儿肠道菌群结构显著改变,菌群丰富度指数(Ace指数,Chao1指数和Observed species)显著增加(均P<0.05),beta多样性显著改变(P<0.05)。肠道菌群组成差异分析显示,MDD患儿拟杆菌属、Phocaeicola属和副拟杆菌属等显著减少,而链球菌属、克雷伯菌属和埃希−志贺菌属等显著升高。MDD患儿血清中前炎症因子IL-1β、IL-4、IL-8、IL-17、TNF-α以及趋化因子IP-10、MCP-1、MIP-1α水平显著升高;而抗炎因子IFN-γ和趋化因子MIP-1β水平及RANTES显著下降(均P<0.05),同时这些细胞因子与肠道关键功能菌具有显著相关性。结论MDD患儿的肠道菌群显著失衡,肠道关键差异菌属与宿主系统性炎症因子显著相关,提示肠道菌群失衡在儿童MDD发生发展中发挥关键作用。Objective To explore the changes in gut microbiota and systemic inflammation among children diagnosed with major depressive disorder(MDD).Methods A total of 39 children newly diagnosed with MDD and 39 healthy children were enrolled.Fecal samples and sera were collected for microbiota analysis and cytokine detection,respectively.Fecal bacterial genomic DNA was extracted for MiSeq high-throughput sequencing,while the inflammatory cytokines in the serum were analyzed using Bio-plex assay.Comparative analyses were conducted to evaluate the differences in microbial community structure and composition,as well as host inflammatory responses,between the MDD children and healthy children.Results The fecal microbiota in children with MDD exhibited significant alterations.Our analysis revealed increased bacterial richness indices(Ace index,Chao1 index,and observed species)(all P<0.05)and significant changes in bacterial beta-diversity(P<0.05)among the children with MDD.The microbiota composition analysis showed that beneficial bacteria such as Bacteroidetes,Phocaeicola and Parabacteroides significantly reduced among children with MDD,while some bacteria including Streptococcus,Klebsiella and Escherichia-Shigella were significantly enriched(all P<0.05).The levels of pro-inflammatory cytokines such as IL-1β,IL-4,IL-8,IL-17,TNF-αand chemokines including IP-10,MCP-1 and MIP-1αwere significantly elevated in children with MDD.Conversely,the anti-inflammatory factors such as IFN-γand the chemokines like MIP-1βand RANTES significantly decreased(all P<0.05).Notably,these altered cytokines were significantly correlated with key functional bacteria in the gut microbiota.Conclusion There is gut dysbiosis in children with MDD,with the predominant differential bacteria being associated with elevated systemic inflammatory cytokines.These findings suggest that gut dysbiosis is a significant factor in the onset and progression of MDD in children.

关 键 词：抑郁症 儿童 系统性炎症 菌群失衡 拟杆菌 

分 类 号：R749.94[医药卫生—神经病学与精神病学]