食管鳞状细胞癌组织中SNAP23的表达及其对食管癌细胞侵袭的影响  

Expression of SNAP23 in esophageal squamous cell carcinoma tissue and its effect on invasion of esophageal cancer cells

作　　者：陈娇 刘清 郑树涛 李慧芳[2] 王薇[3] 刘涛[4] 黄丛改 彭天元 卢晓梅 Chen Jiao;Liu Qing;Zheng Shutao;Li Huifang;Wang Wei;Liu Tao;Huang Conggai;Peng Tianyuan;Lu Xiaomei(Clinical Medical Research Institute,the First Affiliated Hospital of Xinjiang Medical University,State Key Laboratory of Pathogenesis/Prevention and Treatment of High Incidence Diseases in Central Asia,Urumqi 830011,China;Department of Breast Surgery,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China;Department of Gastronenterology,the Affiliated Tumor Hospital of Xinjiang Medical University,Urumqi 830011,China;Department of Clinical Testing Center,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China;Department of Pathology,the Affiliated Hospital of Southwest Medical University,Luzhou 646000,China)

机构地区：[1]新疆医科大学第一附属医院临床医学研究院/省部共建中亚高发病成因与防治国家重点实验室,乌鲁木齐830011 [2]新疆医科大学第一附属医院乳腺外科,乌鲁木齐830011 [3]新疆医科大学附属肿瘤医院消化内科,乌鲁木齐830011 [4]新疆医科大学第一附属医院检验科,乌鲁木齐830011 [5]西南医科大学附属医院病理科,泸州646000

出　　处：《临床与实验病理学杂志》2025年第3期298-303,共6页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(82260568);新疆维吾尔自治区“天山英才”培养计划科技创新领军人才项目(2022TSYCLJ0031);新疆维吾尔自治区自然科学基金重点项目(2022D01D69)。

摘　　要：目的探讨SNAP23在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)组织中的表达及其对ESCC细胞侵袭的影响。方法收集41例ESCC组织及配对的癌旁正常组织(normal adjacent tissue,NAT),采用免疫组化EnVision法检测SNAP23的表达和定位,比较ESCC与NAT组织中SNAP23的差异表达及与临床病理特征的关系。应用Western blot法检测人永生化食管上皮细胞SHEE、ESCC细胞系TE-1、TE-13和KYSE150中SNAP23的表达水平。利用慢病毒载体转染技术,构建SNAP23敲低和过表达的稳定转染细胞株;并利用细胞侵袭实验评估SNAP23对ESCC细胞系TE-13侵袭的影响。结果ESCC组织中的SNAP23呈高表达(53.7%,22/41)显著高于NAT组(19.5%,8/41),差异有统计学意义(χ^(2)=10.303,P<0.01)。ESCC组织中SNAP23的表达水平与肿瘤最大径(χ^(2)=4.193,P<0.05)、浸润深度(χ^(2)=7.264,P<0.05)明显相关,但与患者性别、年龄、发病部位、肿瘤分型、病理分级、脉管瘤栓、神经侵犯和淋巴结转移均无相关性(P>0.05)。ESCC细胞系与人永生化食管上皮细胞SHEE相比,其SNAP23呈高表达(P<0.0001)。与sh-NT组相比,敲低SNAP23表达后,ESCC细胞系TE-13的侵袭能力被抑制(P<0.0001);与Vector组相比,过表达SNAP23后,ESCC细胞系TE-13的侵袭能力增强(P<0.0001)。结论SNAP23在ESCC组织和细胞系中高表达,其在ESCC组织中的表达水平与肿瘤最大径、浸润深度呈正相关;SNAP23促进ESCC细胞的侵袭。Purpose To analyze the expression of SNAP23 in esophageal squamous cell carcinoma(ESCC)and its influence on invasion of ESCC cells.Methods A total of 41 cases of ESCC and paired normal adjacent tissue(NAT)were collected.The expression and localization of SNAP23 were detected by immunohistochemical EnVision method.The differences of SNAP23 expression levels between ESCC tissues and NAT tissues were compared to analyze the relationship between SNAP23 expression and clinicopathological characteristics.The expression level of SNAP23 in human immortalized esophageal epithelial cells SHEE and ESCC cell lines TE-1,TE-13 and KYSE150 were detected by Western blot.Lentiviral vector transfection technique was used to construct stable transfection cell lines with knockdown and overexpression of SNAP23 gene.The effect of SNAP23 on invasion of ESCC cell line TE-13 was evaluated by cell invasion experiment.Results The expression of SNAP23 in ESCC was significantly higher(53.7%,22/41)than that in the NAT group(19.5%,8/41),the difference was statistically significant(χ^(2)=10.303,P<0.01).The expression level of SNAP23 in ESCC tissues was significantly correlated with maximum tumor diameter(χ^(2)=4.193,P<0.05)and invasion depth(χ^(2)=7.264,P<0.05),but was not correlated with patient gender,age,tumor site,tumor type,pathologic grade,vascular embolus,nerve invasion and lymph node metastasis(P>0.05).Compared with human immortalized esophageal epithelial cells SHEE,SNAP23 was highly expressed in ESCC cell lines(P<0.0001).Compared with the sh-NT group,the invasion of ESCC cell line TE-13 was inhibited when SNAP23 expression was knocked down(P<0.0001);compared with the Vector group,the invasion of ESCC cell line TE-13 was enhanced after overexpression of SNAP23(P<0.0001).Conclusion SNAP23 is highly expressed in ESCC tissue and cell lines,and its expression level in ESCC tissues is positively correlated with tumor maximum diameter and invasion depth;SNAP23 promotes the invasion of ESCC cells.

关 键 词：食管肿瘤 鳞状细胞癌 SNAP23 侵袭转移 

分 类 号：R735.1[医药卫生—肿瘤]