转移性结直肠癌中SERPINA4表达与预后的关系  

The relationship between SERPINA4 expression and prognosis in metastatic colorectal cancer

作　　者：高建芳 董晓彤 赵晴 张云香[1,3] 孙慧敏 Gao Jianfang;Dong Xiaotong;Zhao Qing;Zhang Yunxiang;Sun Huimin(Precision Pathology Diagnosis Center,the First Affiliated Hospital of Shandong Second Medical University/Weifang People’s Hospital,Weifang 261041,China;Department of Basic Medicine,Shandong Second Medical University,Weifang 261053,China;Department of Molecular Pathology,Qingdao Central Hospital,University of Health and Rehabilitation Sciences,Qingdao 266011,China)

机构地区：[1]山东第二医科大学第一附属医院/潍坊市人民医院精准病理诊断中心,潍坊261041 [2]山东第二医科大学基础医学院,潍坊261053 [3]康复大学青岛中心医院分子病理科,青岛266011

出　　处：《临床与实验病理学杂志》2025年第3期340-344,351,共6页Chinese Journal of Clinical and Experimental Pathology

基　　金：潍坊市卫生健康委科研项目(WFWSJK-2021-005);潍坊市青年医学人才托举工程[(2024)113]。

摘　　要：目的探讨转移性结直肠癌(colorectal cancer,CRC)中SERPINA4的表达与临床病理特征及预后的关系。方法采用免疫组化EnVision两步法检测104例CRC原发灶、转移灶及癌旁正常肠黏膜组织中SERPINA4蛋白的表达,并分析其与临床病理特征的关系。应用qRT-PCR法检测11对CRC转移灶及配对原发灶石蜡组织中SERPINA4 mRNA的表达。随访患者的生存情况,运用Kaplan-Meier法分析SERPINA4的表达与预后的关系。结果免疫表型:SERPINA4的强阳性率在癌旁正常肠黏膜组织中为97.10%,原发灶为71.20%,转移灶为39.40%,三者之间差异有统计学意义(P<0.001)。qRT-PCR检测结果显示:CRC转移灶中SERPINA4 mRNA低表达(P<0.05)。转移灶中SERPINA4的表达与肿瘤组织学分级、N分期和M分期均有相关性(P<0.05),与患者年龄、性别以及肿瘤发生部位和T分期均无相关性(P>0.05)。SERPINA4高表达的转移性CRC患者生存期高于低表达者。结论SERPINA4表达对转移性CRC患者的生存期和预后判断有重要意义。Purpose To investigate the relationship between SERPINA4 expression and clinicopathological features and prognosis in metastatic colorectal cancer(CRC).Methods EnVision two-step immunohistochemical method was used to detect the expression of SERPINA4 protein in 104 cases of primary and metastatic tumor paraffin tissues of metastatic colorectal cancer and adjacent intestinal normal mucosa,and its relationship with clinicopathological features of patients was analyzed.qRT-PCR was used to detect the expression of SERPINA4 mRNA in 11 pairs of metastatic CRC metastases and paired primary tumor paraffin-embedded tissue samples.The survival of patients was followed up,and the relationship between SERPINA4 expression and patient prognosis was analyzed by Kaplan-Meier method.Results Immunohistochemical results showed that the strong positive expression rate of SERPINA4 was 97.10%in normal mucosa,71.20%in primary lesions,and 39.40%in metastatic lesions.The difference between the three groups was statistically significant(P<0.001).qRT-PCR results showed that SERPINA4 mRNA was lowly expressed in metastatic lesions of metastatic CRC(P<0.05).The expression of SERPINA4 in metastatic lesions was significantly correlated with histological grade,N stage and M stage of the tumor(P<0.05),but not significantly correlated with age,gender,tumor location and T stage of patients(P>0.05).The survival time of patients with metastatic colorectal cancer with high expression of SERPINA4 was longer than that of patients with low expression.Conclusion The expression of SERPINA4 is of great significance in the survival and prognosis of patients with metastatic CRC.

关 键 词：结直肠肿瘤 转移 SERPINA4 预后 

分 类 号：R735.3[医药卫生—肿瘤]