人参皂苷Rg3调控神经元PAS结构域蛋白2影响宫颈癌细胞生物学行为的机制研究  

Effect of ginsenoside Rg3 on PAS domain protein 2 on biological behavior of cervical cancer cells

作　　者：高雅丽[1] 赵子申 高雅梅[3] 宋丽娜[1] 王志聪 GAO Ya-li;ZHAO Zi-shen;GAO Ya-mei(Cangzhou Central Hospital,Cangzhou 061017,China)

机构地区：[1]沧州市中心医院放疗科,河北沧州061017 [2]沧州市人民医院皮肤科,河北沧州061002 [3]沧州市中心医院糖尿病内分泌科,河北沧州061017

出　　处：《中国中西医结合外科杂志》2025年第2期271-276,共6页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：河北省卫生健康委员会医学科学研究课题计划项目(20220344)。

摘　　要：目的:探究人参皂苷Rg3调控神经元PAS结构域蛋白2(NPAS2)影响宫颈癌细胞生物学行为的机制。方法:常规培养宫颈癌Hela细胞,用RNA pull-down和RNA免疫沉淀(RIP)法检测长链非编码RNA X无活性特异性转录本(LncRNA XIST)与NPAS2之间是否存在相互作用;用不同浓度的人参皂苷Rg3(0、25、50、100和200μmol/L)处理Hela细胞,采用MTT法检测细胞增殖率,Transwell法检测细胞侵袭力,流式细胞术检测细胞凋亡,并用RT-PCR检测细胞LncRNA XIST的相对表达量,RT-PCR和Western blot检测细胞NPAS2的mRNA和蛋白相对表达量。结果:在Hela细胞中发现LncRNA XIST能与NPAS2存在相互作用;与空白对照(0μmol/L)组相比,25μmol/L、50μmol/L、100μmol/L及200μmol/L人参皂苷Rg3组细胞增殖率、侵袭力明显减低,细胞凋亡水平明显升高(P<0.05),LncRNA XIST表达降低,NPAS2 mRNA和蛋白表达均升高,并呈剂量依赖性(P<0.05)。结论:人参皂苷Rg3能降低LncRNA XIST表达,促进NPAS2表达,进而影响宫颈癌细胞增殖、侵袭和凋亡等生物学行为。Objective To explore the mechanism of PAS domain protein 2(NPAS2)regulated by ginsenoside Rg3 on the biological behavior of cervical cancer cell.Methods Cervical cancer cell Hela was cultured regularly,and the interaction between LncRNA XIST and NPAS2 was detected by RNA pull-down and RIP.Cervical cancer cells Hela were then treated with ginsenoside Rg3(0,25,50,100 and 200μmol/L)at different concentrations.Cell proliferation rate was detected by MTT assay,cell invasion was detected by Transwell,cell apoptosis was detected by flow cytometry,and the relative expression of LncRNA XIST was detected by RT-PCR.The relative expression of NPAS2 was detected by RT-PCR and Western blot.Results LncRNA XIST was found to interact with NPAS2 in Hela cells.Compared with the blank control group,the proliferation rate and invasion of ginsenoside Rg3 groups in 25μmol/L,50μmol/L,100μmol/L and 200μmol/L groups were significantly decreased,the apoptosis level was significantly increased(P<0.05).The expression of LncRNA XIST was decreased.The mRNA and protein expressions of NPAS2 were increased in a dose-dependent manner(P<0.05).Conclusion Ginsenoside Rg3 can decrease the expression of LncRNA XIST,promote the expression of NPAS2,and then affect the biological behavior of cervical cancer cell proliferation,invasion and apoptosis.

关 键 词：人参皂苷RG3 长链非编码RNA X无活性特异性转录本 神经元PAS结构域蛋白2 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]