GPR15与FOXP3在结直肠癌组织中的表达及其临床预后价值研究  

Expression and clinical prognostic value study of GPR15 and FOXP3 in colorectal carcinoma

作　　者：孙晶璐 童利 王娜娜 吴阳阳 吴琼 Sun Jinglu;Tong Li;Wang Nana;Wu Yangyang;Wu Qiong(Clinicopathological Center,Anhui Public Health Clinical Center(North District,The First Affiliated Hospital of Anhui Medical University),Hefei 230011;Dept of Pathology,Zhenjiang First People’s Hospital,Zhenjiang 212002;Dept of Pathology,Bengbu Medical University,Bengbu 233030;Dept of Pathology,The First Affiliated Hospital of Bengbu Medical University,Bengbu 233004)

机构地区：[1]安徽省公共卫生临床中心(安徽医科大学第一附属医院北区)临床病理中心,合肥230011 [2]镇江市第一人民医院病理科,镇江212002 [3]蚌埠医科大学病理学教研室,蚌埠233030 [4]蚌埠医科大学第一附属医院病理科,蚌埠233004

出　　处：《安徽医科大学学报》2025年第3期480-487,共8页Acta Universitatis Medicinalis Anhui

基　　金：安徽省高校自然科学研究项目(编号:2022AH051505)。

摘　　要：目的探讨GPR15与FOXP3在结直肠癌(CRC)组织中的表达情况及其临床预后价值。方法收集CRC患者根治性手术标本132例,对照组选择距癌灶5 cm以上边缘的正常黏膜组织,采用免疫组织化学技术(Envision二步法)分别检测GPR15与FOXP3在CRC组织及癌旁组织中的表达水平并分析两者与CRC各临床病理因素的关系。采用Kaplan-Meier法绘制生存曲线,分析CRC组织中GPR15与FOXP3的表达与患者生存预后的关联性。采用Cox回归分析CRC患者预后的影响因素。结果免疫组织化学结果显示,CRC组织中GPR15与FOXP3两者的表达水平均显著高于正常结直肠黏膜组织(P<0.05)。GPR15在CRC组织中的表达与部位、神经侵犯、TNM分期相关,FOXP3表达与性别相关(P<0.05)。两者的表达均与患者年龄、肿块大小、分化程度、组织分型、浸润深度、肿瘤出芽、脉管侵犯、淋巴结转移等临床病理特征无显著相关性。相关性分析表明,GPR15与FOXP3两者的表达情况无显著相关性(Kappa系数=-0.019)。GPR15阳性组生存预后显著劣于阴性组(log-rank检验:χ^(2)=4.3,P=0.039);FOXP3阳性组生存预后显著优于阴性组(log-rank检验:χ^(2)=7.3,P=0.007)。年龄≤55岁、GPR15阳性、FOXP3阴性是促使CRC患者预后不良的独立危险因素(P<0.05)。结论GPR15与FOXP3均在CRC组织中的表达水平明显高于癌旁组织,GPR15与FOXP3有望成为CRC早期筛查、精准治疗、预后评估的新型肿瘤标志物。Objective To investigate the expressions of GPR15 and FOXP3 in colorectal carcinoma(CRC)tissues and their clinical prognostic values.Methods A total of 132 patients with CRC underwent radical surgery were collected.The control group selected the normal mucosal tissues more than 5 cm away from the edge of the cancer focus.Immunohistochemistry(Envision two-step method)was used to detect the expression levels of GPR15 and FOXP3 in CRC and adjacent tissues,and analyze their relationships with clinicopathological factors of colorectal cancer.Kaplan-Meier method was used to draw the survival curve to analyze the correlation between the expressions of GPR15 and FOXP3 and the survival prognosis of patients with CRC.The factors influencing prognosis of patients with colorectal cancer were analyzed by Cox regression.Results The immunohistochemistry showed that the expression levels of GPR15 and FOXP3 in CRC were significantly higher than those in normal colorectal mucosal tissues(P<0.05).The expression of GPR15 in CRC tissues was correlated with location,nerve invasion and TNM stage;FOXP3 expression was correlated with sex(P<0.05).Both expressions were not significantly correlated with the clinicopathologic features of age,tumor size,differentiation degree,tissue type,depth of invasion,tumor budding,vascular invasion and lymph node metastasis.Correlation analysis showed that there was no significant correlation between GPR15 and FOXP3 expression(Kappa=-0.019,P>0.05).The survival prognosis of GPR15 positive group was significantly worse than that of negative group(log-rank:χ^(2)=4.3,P=0.039);while the survival prognosis of FOXP3 positive group was significantly better than that of negative group(log-rank:χ^(2)=7.3,P=0.007).Age≤55 years,positive GPR15 and negative FOXP3 were independent risk factors for poor prognosis in patients with CRC(P<0.05).Conclusion The expression levels of GPR15 and FOXP3 in CRC are significantly higher than those in paracancer tissues,GPR15 and FOXP3 are expected to become new tumor markers for

关 键 词：结直肠肿瘤 GPR15 FOXP3 免疫组织化学 预后 

分 类 号：R735.3[医药卫生—肿瘤]