小麦DH系创制中培养基、秋水仙浓度的筛选及其HMW-GS组成分析  

Screening of Culture Medium and Concentration of Colchicine for Construction of Wheat DH Lines and Their HMW-GS Composition Analysis

作　　者：毕珍珍 郑艳 李辉 李晓建 刘雯 母学荣 朱娜 郑佳乐 宋瑜龙[1] 闵东红[1] 张小红[2] BI Zhenzhen;ZHENG Yan;LI Hui;LI Xiaojian;LIU Wen;MU Xuerong;ZHU Na;ZHENG Jiale;SONG Yulong;MIN Donghong;ZHANG Xiaohong(College of Agronomy,Northwest A&F University/State Key Laboratory of Crop Stress Biology in Arid Area,Yangling,Shaanxi 712100,China;College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《麦类作物学报》2025年第4期432-439,共8页Journal of Triticeae Crops

基　　金：科技创新2030-重大项目(2023ZD0402601)。

摘　　要：为了提高小麦DH系创制效率,以11个小麦杂交组合的F1代为材料,比较分析了两种培养基(NPB-99和CHB)下小麦花药的诱导效率以及三种浓度秋水仙素处理(4、6和8 g·L^(-1))下单倍体的染色体加倍效率,并利用SDS-PAGE对获得的DH株系进行优质亚基检测。结果表明,CHB培养基花药诱导效率优于NPB-99培养基。在三种秋水仙素浓度中,6 g·L^(-1)诱导单倍体加倍的效率最高。在所获得的33个DH系中共鉴定出16个株系携带优质亚基组合(1/7+8/5+10或1/7+9/5+10),且具有较好的烘焙品质,可作为小麦品质育种的种质资源。In order to improve the construction of wheat DH lines,the F 1 generation of 11 wheat hybrid combinations was used as materials to compare and analyze the induction efficiency of wheat anthers under two media(NPB-99 and CHB)and the chromosome doubling efficiency of haploids under three concentrations of colchicine treatments(4,6 and 8 g·L^(-1)).The high-molecular-weight glutenin subunits(HMW-GS)of the obtained DH lines were identified by SDS-PAGE.The results showed that the anther induction efficiency of CHB medium was better than that of NPB-99 medium.The concentration of 6 g·L^(-1)colchicine was more efficient in inducing haploid doubling.A total of 16 lines carrying prior HMW-GS combinations(1/7+8/5+10 or 1/7+9/5+10)were identified from the 33 DH lines,with good baking quality,which could provide genetic resources for subsequent wheat quality breeding research.

关 键 词：小麦 花药培养 DH系 HMW-GS 

分 类 号：S512.1[农业科学—作物学] S330