基于MOF-聚多巴胺纳米复合材料的制备及体外抗肿瘤活性研究  

Preparation and Antitumor Activity of MOF-Polydopamine Nanocomposites in Vitro

作　　者：李雯雯 郭漫雯 周言 杨雪 黄婵琦 高诗婷 薛莹雪 LI Wenwen;GUO Manwen;ZHOU Yan;YANG Xue;HUANG Chanqi;GAO Shiting;XUE Yingxue(College of Pharmacy,Jilin Medical University,Jilin City,Jilin Province 132013,China)

机构地区：[1]吉林医药学院药学院,吉林吉林132013

出　　处：《吉林医药学院学报》2025年第2期97-101,105,共6页Journal of Jilin Medical University

基　　金：吉林省科技发展计划项目(YDZJ202401025ZYTS);吉林市科技创新发展计划项目(20230103033);大学生创新创业训练计划项目(202213706001,S202313706016)。

摘　　要：目的构建一种具有光热-化学动力学-化疗协同治疗肿瘤的纳米材料,提高肿瘤治疗效果。方法采用金属有机框架(metal-organic framework,MOF)为药物载体,负载药物阿霉素(DOX),表面包裹聚多巴胺(PDA),制备出Fe-MOF@DOX@PDA纳米材料。通过透射电镜和动态光散射对Fe-MOF@DOX@PDA纳米粒子的形貌及稳定性进行表征。通过红外激光器考察Fe-MOF@DOX@PDA纳米粒子的光热转换效果。通过紫外-可见吸收光谱考察纳米粒子的药物释放行为以及羟基自由基(·OH)的生成效果。利用MTT方法考察Fe-MOF@DOX@PDA纳米粒子抑制肝癌HepG2细胞增殖的效果。结果Fe-MOF@DOX@PDA纳米粒子具有良好的光热稳定性。纳米粒子与H_(2)O_(2)作用可以产生·OH。Fe-MOF纳米粒子对DOX的装载效率可达到74%。药物在pH=5.0的条件下释放率更高。对比不同的治疗组,Fe-MOF@DOX@PDA纳米粒子+H_(2)O_(2)+光照组对HepG2细胞的抑制效果是最好的。结论Fe-MOF@DOX@PDA纳米粒子可以作为理想的光热-化学动力学-化疗协同治疗试剂,有效提高肿瘤治疗效果。Objective To construct a kind of nanomaterial with photothermal-chemodynamic-chemo synergistic treatment for tumor and improve the therapeutic effect.Methods Fe-MOF@DOX@PDA nanomaterial was prepared by using metal-organic framework(MOF)as drug carrier loaded with doxorubicin(DOX)and coated with polydopamine(PDA).The morphology and stability of Fe-MOF@DOX@PDA nanoparticles were characterized by TEM and DLS.The photothermal conversion effect of Fe-MOF@DOX@PDA nanoparticle was investigated by infrared laser.The drug release behavior of the nanoparticle and the production of·OH were investigated by UV-vis.The inhibitory effect of Fe-MOF@DOX@PDA nanoparticle on hepatoma HepG2 cell proliferation was investigated by MTT method.Results Fe-MOF@DOX@PDA nanoparticles were of good photothermal effect.·OH can be produced by the interaction of the nanoparticle with H_(2)O_(2).The loading efficiency of Fe-MOF nanoparticle for DOX was 74%.The drug release rate was higher at pH 5.0.Compared with different treatment groups,Fe-MOF@DOX@PDA nanoparticle+H_(2)O_(2)+light group showed the best inhibitory effect on HepG2 cells.Conclusion Fe-MOF@DOX@PDA nanoparticle can be used as an ideal photothermal-chemodynamic-chemo synergistic therapeutic reagent,which can effectively improve the therapeutic effect of tumor.

关 键 词：金属有机框架 纳米复合材料 抗肿瘤 光热 化学动力学 

分 类 号：R730[医药卫生—肿瘤]