虎杖苷对放射性脑损伤小鼠的保护作用  

Protective effect of polydatin on mice with radiation induced brain injury

作　　者：孔蔺莎 罗慧萍 张潇月 谈峰榕 蒙文宇 阮林[3] 韦力[1,4] KONG Linsha;LUO Huiping;ZHANG Xiaoyue;TAN Fengrong;MENG Wenyu;RUAN Lin;WEI Li(School of Basic Medicine,Guangxi Medical University,Nanning 530021,Guangxi,China;Department of Anesthesiology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China;Department of Radiotherapy,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China;Guangxi Key Laboratory of Human Development and Disease Research,Nanning 530000,Guangxi,China)

机构地区：[1]广西医科大学基础医学院,广西南宁市530021 [2]广西医科大学第一附属医院麻醉科,广西南宁市530021 [3]广西医科大学第一附属医院放疗科,广西南宁市530021 [4]广西高校人体发育与疾病研究重点实验室,广西南宁市530000

出　　处：《广西医学》2025年第2期237-243,共7页Guangxi Medical Journal

基　　金：广西自然科学基金(2024GXNSFAA010200)。

摘　　要：目的 探讨虎杖苷对放射性脑损伤小鼠的保护作用。方法 将40只小鼠随机分为对照组、虎杖苷组、放射组、放射+虎杖苷组,每组10只。给予放射组、放射+虎杖苷组小鼠20 Gy的^(60)COγ射线照射,给予对照组和虎杖苷组0 Gy的^(60)COγ射线照射,共照射7 d,照射期间每天给予虎杖苷组和放射+虎杖苷组小鼠腹腔注射100 mg/kg的1%虎杖苷溶液,对照组和放射组小鼠腹腔注射等体积生理盐水。使用Morris水迷宫实验评估各组小鼠的学习记忆功能,采用苏木素?伊红染色观察小鼠脑组织的病理学改变情况,采用免疫组织化学染色法检测小鼠脑组织小胶质细胞阳性突触数,小胶质细胞、星形胶质细胞阳性细胞胞体面积,纤维蛋白原(FIB)、CD31的表达情况及微血管数量。结果 与照射前相比,照射后放射组小鼠逃避潜伏期延长、穿越平台次数减少(P<0.05),运动轨迹为随机性。照射后,与对照组相比,放射组小鼠的逃避潜伏期延长、穿越平台次数减少(P<0.05),运动轨迹为随机性;与放射组相比,放射+虎杖苷组小鼠的逃避潜伏期缩短、穿越平台次数增加(P<0.05),运动轨迹更加集中于目标象限。与对照组相比,放射组小鼠脑组织结构紊乱,细胞核出现明显固缩,血管数量减少;与放射组相比,放射+虎杖苷组小鼠脑组织结构紊乱有明显改善。与对照组相比,放射组小鼠脑组织小胶质细胞胞体面积增加、微血管数量减少、血管内FIB沉积增多、CD31表达下降(P<0.05);与放射组相比,放射+虎杖苷组小鼠脑组织小胶质细胞胞体面积减小、突触数量减少、星形胶质细胞胞体面积减小、微血管数量增多、血管内FIB沉积减少、CD31表达升高(P<0.05)。结论 虎杖苷可通过减轻放射性脑损伤小鼠神经炎症反应,提高脑血管的完整性,从而减轻学习记忆功能障碍,恢复脑神经系统功能。Objective To investigate the protective effect of polydatin on mice with radiation induced brain injury.Methods A total of 40 mice were randomly assigned to control group,polydatin group,radiation group,or radiation+polydatin group,with 10 mice in each group.Mice of the radiation and radiation+polydatin groups received ^(60)COγirradiation in 20 Gy,whereas the control group and the polydatin group received ^(60)COγirradiation in 0 Gy,for a 7⁃day irradiation.During irradiation,mice of the polydatin and radiation+polydatin groups received intraperitoneal injection of 1%polydatin solution in 100 mg/kg,while mice of the control group and the radiation group received intraperitoneal injection of normal saline in equivalent volume.The Morris water maze test was adopted to evaluate learning and memory function of mice in various groups.The hematoxylin⁃eosin staining was employed to observe pathological changes of brain tissues in mice.The immunohistochemical staining method was used to detect number of microglial positive synapse,and cell body areas of microglial cells,astrocytes,as well as expressions of fibrinogen(FIB),and CD31,and number of microvessels in brain tissues of mice.Results Compared with before irradiation,mice of the radiation group obtained prolonged escape latency and reduced platform⁃crossing times after irradiation(P<0.05),and the motion trajectory was random.After irradiation,compared with the control group,mice of the radiation group acquired prolonged escape latency and reduced platform⁃crossing times(P<0.05),and the motion trajectory was random;furthermore,compared with the radiation group,mice of the radiation+polydatin group presented as shortened escape latency and increased platform⁃crossing times(P<0.05),and the motion trajectory was more focused on the target quadrant.Compared with the control group,mice in the radiation group presented as disordered brain tissue structure,obvious nucleus contraction,decreased number of vessels;in addition,compared with the radiation group,mice i

关 键 词：放射性脑损伤 虎杖苷 学习记忆功能 小胶质细胞 星形胶质细胞 小鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]