层次分析法结合响应面法优化石榴皮的提取工艺  

The Extraction Process of Pomegranate Peelwas Optimized by Analytic Hierarchy Process and Response Surface Methodology

作　　者：陈振东 施洋 谭雪 时欢欢 单新 韩瑞 CHEN Zhendong;SHI Yang;TAN Xue;SHI Huanhuan;SHAN Xin;HAN Rui(Integrated Chinese and Western Medicine Hospital of Karamay(Karamay Municipal Peoples Hospital),Karamay Xinjiang 834000,China)

机构地区：[1]克拉玛依市中西医结合医院/克拉玛依市人民医院,新疆克拉玛依834000

出　　处：《中医药导报》2025年第3期60-64,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：中国药学会全国医药经济信息网科普重点研究项目(CMEI2024KPYJ00109);新疆维吾尔自治区重点实验室(2016D03026)。

摘　　要：目的:采用层次分析法和响应面法优化石榴皮的提取工艺。方法:采用高效液相色谱法phenmomenexsynergiTM(250.0 mm×4.6 mm,4.0μm),甲醇(A)-0.15%三氟乙酸(B)为流动相,梯度洗脱,流速为1.0 mL/min,柱温30℃,检测波长320 nm,进样量10μL。在单因素优选的基础上结合响应面法考察提取次数、乙醇体积分数、提取时间3个主要因素,以安石榴苷、没食子酸、绿原酸的含量及出膏率为考察指标,层次分析法计算权重系数,通过综合评分优选石榴皮的提取工艺。结果:安石榴苷、没食子酸、绿原酸线性范围分别为6.4144~19.2432μg/mL(r=0.9999),3.2168~9.6504μg/mL(r=0.9999),3.2104~9.6312μg/mL(r=0.9999),精密度、稳定性、重复性试验RSD值均小于2%;平均加样回收率(n=6)分别为99.63%(RSD=1.52%)、99.16%(RSD=1.48%)、100.90%(RSD=1.25%);石榴皮的提取工艺为70%乙醇提取2次,每次1 h。结论:优化的工艺提取条件简便,重现性良好,适用于石榴皮药材的提取。Objective:The extraction process of pomegranate peel was optimized by analytic hierarchy process(AHP)and response surface methodology.Methods:The chromatographic separation was achieved on an phenmomenexsynergiTM(250.0 mm×4.6 mm,4.0μm)column with methanol(A)-0.15%trifluoroacetic acid aqueous solution(B)as mobile phase for gradient elution,at the flow rate of 1.0 mL/min.The column temperature was 30℃.The determination wavelength was 320 nm,and the injection volume was 10μL.On the basis of univariate optimum seeking method the three main factors of extraction times,ethanol concentration and extraction time were investigated by response surface methodology.With the content and the extract rate of punicalagin,gallic acid and chlorogenic acid as the indexes,the weight coefficient was calculated by AHP method.Then the extraction process of Pomegranate peel was optimized by comprehensive score.Results:The linear rang of punicalagin,gallic acid and chlorogenic acid were 6.4144-19.2432μg/mL(r=0.9999),3.2168-9.6504μg/mL(r=0.9999),3.2104-9.6312μg/mL(r=0.9999),respectively.The RSD of the precision,stability and repeatability tests were all less than 2%.The average simple recovery rate of punicalagin,gallic acid and chlorogenic acid were 99.63%(RSD=1.52%),99.16%(RSD=1.48%),100.90%(RSD=1.25%),respectively.The extraction process of Pomegranate peel was as follows,extraction with 70%ethanol for 2 times,1 hour each time.Conclusion:The optimized extraction conditions is simple and reproducible,which is suitable for the extraction of pomegranate peel.

关 键 词：石榴皮 提取工艺 层次分析法 响应面设计 含量测定 

分 类 号：R284.2[医药卫生—中药学]