蚯蚓肠道铁还原过程对活性氧形成的影响  

Effect of intestinal iron reduction process on reactive oxygen species formation in earthworm

作　　者：张颖 高泽萍 王旭 李顺顺 黄涛[1,2] 周国伟 WANG Xu;LI Shun-shun;HUANG Tao;ZHOU Guo-wei(College of Resource and Environmental Engineering,Anhui University,Hefei 230601,China;Anhui Provincial Key Laboratory of Wetland Ecological Protection and Restoration,Anhui University,Hefei 230601,China)

机构地区：[1]安徽大学资源与环境工程学院,安徽合肥230601 [2]湿地生态保护与修复安徽省重点实验室,安徽合肥230601

出　　处：《中国环境科学》2025年第4期2053-2062,共10页China Environmental Science

基　　金：国家自然科学基金资助项目(42107143)。

摘　　要：以威廉环毛蚓为实验对象,借助原位测定和微宇宙培养实验,结合高通量测序和生化分析展开研究.结果显示,葡萄糖处理组中Fe(Ⅱ)生成量最多,氨基酸组最少.铁还原过程中,表面吸附态Fe(Ⅱ)含量最高,为0.6~24.38mmol/L;离子态Fe(Ⅱ)最低,0.02~2.21mmol/L.铁还原菌群落结构受有机质类型影响显著,不同处理组中优势铁还原菌各不相同.此外,铁还原过程伴随着活性氧自由基(ROS)的产生,其中过氧化氢(H_(2)O_(2))含量最高,为0.32~0.73mmol/L,与离子态Fe^(2+)、表面吸附态Fe(Ⅱ)、高结晶态铁含量呈显著正相关,而与有机物络合态铁含量呈显著负相关;羟基自由基(·OH)与离子态Fe^(2+)、吸附态Fe(Ⅱ)、高结晶态铁呈显著正相关;超氧阴离子(O_(2)^(·-))与低结晶态铁呈显著正相关,与吸附态Fe(Ⅱ)、高结晶态铁呈显著负相关.研究结果为了解蚯蚓在土壤铁循环和ROS形成过程中的作用提供了新视角,对于利用ROS控制和降解污染物具有潜在应用价值.Earthworm(Pheretima guillelmi)was selected as the model animal.Combination of in situ determination,high-throughput sequencing and biochemical analysis,the results indicated that the production of Fe(Ⅱ)was highest in the glucose treatment group and lowest in the amino acid setup.During the iron(Ⅲ)reduction process,the content of surface-adsorbed Fe(Ⅱ)was the highest,ranging from 0.6 to 24.38mmol/L;whereas the content of ionic Fe(Ⅱ)was the lowest,ranging from 0.02 to 2.21mmol/L.The community structure of iron(Ⅲ)-reducing bacteria was significantly influenced by the type of organic matter,and the dominant iron(Ⅲ)-reducing bacteria in different treatment groups were diverse.Additionally,the iron(Ⅲ)reduction process was accompanied by the generation of reactive oxygen species(ROS).The content of hydrogen peroxide(H_(2)O_(2))was the highest,ranging from 0.32 to 0.73mmol/L,and be show a significant positive correlation with the contents of ionic Fe^(2+),surface-adsorbed Fe(Ⅱ),and high-crystalline iron,while exhibiting a significant negative correlation with the content of iron in organic complex state.Hydroxyl radical(·OH)has a significant positive correlation with ionic Fe^(2+),adsorbed Fe(Ⅱ),and high-crystalline iron.Superoxide anion(O_(2)^(·-))has a significant positive correlation with low-crystalline iron and a significant negative correlation with adsorbed Fe(Ⅱ)and high-crystalline iron.The research results provide a new perspective for understanding the role of earthworms gut microbiota in soil iron cycling and ROS formation,which can be applied for pollutant control and degradation.

关 键 词：蚯蚓肠道菌群 铁还原 有机质 活性氧 

分 类 号：X172[环境科学与工程—环境科学]