微生物插入序列的多样性及其与功能基因的共现  

Diversity of microbial insertion sequences and their co-occurrence with functional genes

作　　者：钟文婧 苏建强[1,2] 李虎 周艳艳[1,3] ZHONG Wen-jing;SU Jian-qiang;LI Hu;ZHOU Yan-yan(State Key Laboratory of Regional and Urban Ecology,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021,China;University of Chinese Academy of Sciences,Beijing 100049,China;Guangdong Provincial Key Laboratory of Marine Biotechnology,Shantou University,Shantou,515063,China)

机构地区：[1]区域与城市生态安全全国重点实验室,中国科学院城市环境研究所,福建厦门361021 [2]中国科学院大学,北京100049 [3]广东省海洋生物技术重点实验室,汕头大学,广东汕头515063

出　　处：《中国环境科学》2025年第4期2314-2322,共9页China Environmental Science

基　　金：国家自然科学基金资助项目(42161134002);广东省海洋生物技术重点实验室资助项目(GPKLMB202204)。

摘　　要：基于ISfinder数据库,结合文献检索,对5812种插入序列(IS)的多样性及其与功能基因的共现关系进行了系统分析.研究表明,不同IS家族(IS family)在宿主中的分布以及与功能基因的共现特征存在差异.DDE转座酶类型IS占据主导地位,IS5和IS3家族涵盖的IS种类最多,而ISH6最少.IS广泛存在于细菌和古菌中,多个IS家族具有宿主特异性,仅发现于细菌或古菌.进一步分析发现IS与多种功能基因(如抗生素抗性、重金属抗性、应激抗性等)共现,显示出它们在环境适应和微生物抗性基因传播中的重要作用.部分IS具有与多种功能基因共现的特性,展现了更广泛的生态适应性;而另一些IS的共现基因则功能相对专一.实验验证IS在基因传播和宿主适应中的机制是未来研究重点.这不仅有助于理解微生物如何适应环境变化、应对选择压力,还能为抗生素抗性、病原体传播等公共卫生问题提供理论基础,从而为发展新的防控策略和生物技术应用奠定基础.Based on the ISfinder database and reference meta-analysis,this study conducted a systematic study of the diversity of 5812 insertion sequences(ISs)and their co-occurrence with functional genes.The study found significant differences in the distribution of different IS families among hosts,as well as in their co-occurrence with functional genes.DDE-type ISs are predominant,with the IS5 and IS3 families containing the most ISs,while the ISH6 family contains the fewest.ISs are widely found in bacteria and archaea,and several IS families show host specificity,being found only in either bacteria or archaea.The study demonstrated that IS co-occur with various functional genes,such as antibiotic resistance,heavy metal resistance,and stress resistance,indicating their significant role in environmental adaptation and the spread of antibiotic resistance genes among pathogens.Some IS exhibited co-occurrence with multiple functional genes,suggesting broader ecological adaptability,while others showed functional specificity.Future research should focus on experimentally validating the mechanisms through which IS mediate gene transfer and host adaptation,to reveal the mechanism of microbial evolution and ecological adaptation.

关 键 词：插入序列 ISfinder数据库 多样性 功能基因 共现 

分 类 号：Q933[生物学—微生物学] X172[环境科学与工程—环境科学]