多基因联用技术在虫草研究中应用进展  

Advance in Application of Multigene Analysis Technology in Cordyceps Research

作　　者：朱新燕 鲍素敏 李春如 李增智 ZHU Xinyan;BAO Sumin;LI Chunru;LI Zengzhi(Zhejiang BioAsia Life Science Institute,Jiaxing 314200,Zhejiang,China;Dongguan HEC Biopharmaceutical R&D Co.,Ltd.,Dongguan 523000,Guangdong,China)

机构地区：[1]浙江泛亚生命科学研究院,浙江嘉兴314200 [2]东莞市东阳光生物药研发有限公司,广东东莞523000

出　　处：《食用菌学报》2025年第2期104-114,共11页Acta Edulis Fungi

摘　　要：对热休克蛋白基因(hsp70)、RNA聚合酶基因(rpb1和rpb2)、交配型基因(mat-alpha和matHMG)、18SrDNA(nrSSU)、28SrDNA基因(nrLSU)、ITS序列、真核翻译延伸因子(ef-1α)、微管蛋白基因(β-tubulin)、线粒体细胞色素基因(cytb和coI)和丝氨酸蛋白基因(csp1)在真核生物中的家族地位、调控作用、基因蛋白编码结构特征等进行简介;并描述虫草类真菌相关的多基因扩增引物名称、碱基序列和片段长度。多基因联用技术能明确虫草的地理谱系起源、亲缘关系和寄主的遗传分化,为确立种属的分类地位和发现新的种属提供方法,为在分子水平鉴别虫草的真伪提供参考。笔者对近年来应用多基因联用分析技术在虫草(Cordyceps)系统发育等方面取得的研究进展进行归纳、总结和展望。Multigene analysis technology has been used to clarify the geographical lineage origin, genetic relationship and genetic differentiation of hosts of Cordyceps spp., and thus provides a method for establishing taxonomic status, discovering new species and genera, and identifying the authenticity of Cordyceps spp.at the molecular level. Research progress in the phylogeny of Cordyceps spp. by using multi-gene linkage analysis technology in recent years was summarized, discussed and prospected.The family status, regulatory role and structural characteristics of heat shock protein gene(hsp70), RNA polymerase genes(rpb1 and rpb2),mating type genes(mat-alpha and mat-HMG), 18SrDNA(nrSSU), 28SrDNA(nrLSU), ITS gene, Eukaryotic translation elongation factor(ef-1α), beta-tubulin gene(β-tubulin), mitochondrial cytochrome genes(cytb and coI) and serine protein gene(csp1) in eukaryotes were reviewed. Primer name, base sequence and fragment length for multigene amplification in cordycipitoid fungi were described.

关 键 词：虫草 多基因 引物 系统进化 

分 类 号：S567.3[农业科学—中草药栽培]