hsa_circRNA_0073762促进甲状腺乳头状癌细胞侵袭、转移和增殖的机制研究  

Mechanism of hsa_circRNA_0073762 in promoting invasion,metastasis,and proliferation of papillary thyroid carcinoma cells

作　　者：贾恒哲 张波林 张杰[1] 吴振宇 姜霞[1] 田延锋[1] JIA Heng-zhe;ZHANG Bo-lin;ZHANG Jie;WU Zhen-yu;JIANG Xia;TIAN Yan-feng(Department of General Surgery/Breast and Thyroid Diagnosis and Treatment Center,the First Hospital of Hebei Medical University,Shijiazhuang 050031,China)

机构地区：[1]河北医科大学第一医院普外科,乳腺甲状腺诊疗中心,河北石家庄050031

出　　处：《河北医科大学学报》2025年第4期444-452,共9页Journal of Hebei Medical University

基　　金：河北省重点研发计划项目(21372401D)。

摘　　要：目的探讨hsa_circ_0073762在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)中的调节作用,揭示其具体作用机制。方法采用高通量测序鉴定PTC与正常细胞差异表达环状RNA(circRNA),逆转录-定量聚合酶链式反应(reverse transcription-quantitative polymerase chain reaction,RT-qPCR)验证细胞系及PTC与癌旁组织差异表达,核酸电泳、sanger测序、RNA酶消化实验验证其成环性及稳定性,通过细胞计数试剂盒8(cell counting kit-8,CCK-8)、划痕愈合、克隆形成和迁移实验,分析hsa_circ_0073762对PTC细胞增殖、迁移和侵袭的影响,双荧光素酶报告实验验证hsa_circ_0073762、miR-548c-3p、ENAH基因的相互作用。结果RT-qPCR验证测序结果显示,hsa_circ_0073762在PTC细胞中显著上调(P<0.001),且在癌组织中显著上调(P<0.05)。敲低hsa_circ_0073762可显著抑制PTC细胞的侵袭、转移和增殖能力。生物信息学预测和双荧光素酶报告实验表明hsa_circ_0073762、miR-548c-3p、ENAH构成ceRNA网络(P<0.05)。结论hsa_circ_0073762对PTC侵袭、转移和增殖进程存在影响,可能成为PTC的新型诊断生物标志物和潜在治疗靶点。Objective To investigate the regulatory role of hsa_circ_0073762 in papillary thyroid carcinoma(PTC)and to explore its underlying mechanisms.Methods High-throughput sequencing was used to identify differentially expressed circular RNAs(circRNAs)in thyroid cancer and normal cells.Reverse transcription-quantitative polymerase chain reaction(RT-qPCR)was performed to validate the differential expression between cell lines,PTC,and adjacent tissues.Nucleic acid electrophoresis,sanger sequencing,and RNA enzyme digestion experiments were conducted to verify the circularity and stability of hsa_circ_0073762.Cell counting kit-8(CCK-8),wound healing,clone formation,and migration assays were used to analyze the effects of hsa_circ_0073762 on PTC cell proliferation,migration,and invasion.A dual-luciferase reporter assay was used to validate the interaction between hsa_circ_0073762,miR-548c-3p,and the ENAH gene.Results RT-qPCR validation of sequencing results showed that hsa_circ_0073762 was significantly upregulated in PTC cells(P<0.001)and in cancer tissues(P<0.05).Knockdown of hsa_circ_0073762 significantly inhibited the invasion,migration,and proliferation of PTC cells.Bioinformatics prediction and dual-luciferase reporter assays indicated that hsa_circ_0073762,miR-548c-3p,and ENAH formed a ceRNA network(P<0.05).Conclusion hsa_circ_0073762 has effects on the invasion,migration,and proliferation processes of PTC and may serve as a novel diagnostic biomarker and potential therapeutic target for PTC.

关 键 词：甲状腺癌 乳头状 RNA 环状 肿瘤浸润 

分 类 号：R736.1[医药卫生—肿瘤]