桑叶提取物对黑斑侧褶蛙生长性能、胴体成分、血清生化指标的影响  

Effects of mulberry leaf extract on growth performance,carcass composition and serum biochemical indices of Pelophylax nigromaculatus

作　　者：陈祥 刘东华 耿琳烨 沈诗桀 王倩茹 沈以红[2] 黄先智[2] 段彪[1] CHEN Xiang;LIU Donghua;GENG Linye;SHEN Shijie;WANG Qianru;SHEN Yihong;HUANG Xianzhi;DUAN Biao(College of Fisheries,Southwest University,Chongqing 400716,China;National Key Laboratory for Efficient Breeding and Utilization of Resource Insects,Southwest University,Chongqing 400716,China)

机构地区：[1]西南大学水产学院,重庆400716 [2]西南大学,资源昆虫高效养殖与利用全国重点实验室,重庆400716

出　　处：《水产学报》2025年第4期185-197,共13页Journal of Fisheries of China

基　　金：现代农业产业技术体系建设专项(CARS-18)。

摘　　要：【目的】探究不同水平桑叶提取物对黑斑侧褶蛙生长性能、胴体成分及血清生化指标的影响。【方法】实验以396只健康黑斑侧褶蛙[体重(2.7±0.2)g]为对象,随机分为4组,每组3个重复,每个重复33只,分别饲喂在基础饲料中添加0、3、6、9 g/kg桑叶提取物的实验饲料,饲养周期为56 d。【结果】桑叶提取物组成活率、末均重、增重率和特定生长率呈先升后降趋势,M03组末均重、增重率、特定生长率较对照组分别显著提高4.18%、6.98%、2.86%。摄食率和饵料系数呈先降后升趋势,M03组较对照组分别显著降低7.73%、12.32%,M09组与对照组无显著差异。与对照组相比,M03组、M06组胴体粗蛋白、粗脂肪含量无显著变化,肝脂中的含量显著降低10.17%、10.60%;M09组胴体蛋白质含量显著下降4.11%。随桑叶提取物添加水平升高,M03组TC、LDL较对照组显著降低35.23%、53.12%;M03组、M06组肝脏SOD较对照组显著提高18.59%、9.67%,M09组无显著差异;肝脏T-AOC、MDA均高于对照组,但无显著差异。【结论】桑叶提取物可有效提高黑斑侧褶蛙对饲料的消化利用率、抗氧化能力,促进其生长。实验结果显示,在黑斑侧褶蛙基础饲料中桑叶提取物的添加添加量为0.34%~0.42%。本研究可为黑斑侧褶蛙绿色健康养殖提供理论依据。This study designed gradient concentration of 0(control),3,6,and 9 g/kg mulberry leaf extract(MLE)added to the essential diet and the initial weight of(2.7±0.2)g healthy Pelophylax nigromaculatus was fed for 56 days to investigate the effects of MLE at different levels on growth performance,carcass composition,and serum biochemical indices.The gradient concentration experiment were labelled as C control group,M03 group,M06 group and M09 group,respectively.Survival rate,final body weight(FBW),weight gain rate(WGR),and specific growth rate(SGR)initially increased and then decreased with rising MLE levels.The M03 group showed significantly higher FBW,WGR,and SGR by 4.18%,6.98%,and 2.86%,respectively,compared to the control group(P<0.05).Feed intake rate(FR)and feed conversion ratio(FCR)initially decreased and then increased,with the M03 group exhibiting a significant reduction of 7.73% and 12.32% in FR and FCR(P<0.05).Meanwhile,the M09 group showed no significant difference from the control group(P>0.05).Compared to the control,carcass crude protein and crude lipid in the M03 and M06 groups remained unchanged(P>0.05).The hepatic lipid content significantly decreased by 10.17% and 10.60%(P<0.05).However,carcass protein in the M09 group declined significantly by 4.11%(P<0.05).Serum total cholesterol(TC)and low-density lipoprotein(LDL)in the M03 group decreased significantly by 35.23% and 53.12%(P<0.05).Hepatic superoxide dismutase(SOD)activity in the M03 and M06 groups increased significantly by 18.59% and 9.67%(P<0.05),whereas no significant difference was observed in the M09 group(P>0.05).Hepatic total antioxidant capacity(T-AOC)and malondialdehyde(MDA)levels were higher than the control group but not statistically significant(P>0.05).Dietary MLE supplementation improves feed utilization efficiency,antioxidant capacity and growth performance in P.nigromaculatus.Based on a comprehensive evaluation,the optimal MLE inclusion level in the basal diet was recommended to be 0.34%-0.42%.This study provides a theoretical fo

关 键 词：黑斑侧褶蛙 桑叶提取物 生长性能 胴体成分 血清生化指标 

分 类 号：S963.7[农业科学—水产养殖]