基于蛋白组学分析蓝光对豚鼠眼球正视化过程的影响  

Effects of Blue Light on Emmetropization in Guinea Pigs Based on Proteomic Analysis

作　　者：肖珺心 权卓娅 肖虎 Thomas Cheun Lam 朱敏怡 王丹阳 XIAO Junxin;QUAN Zhuoya;XIAO Hu;LAM Thomas Cheun;ZHU Minyi;WANG Danyang(Department of Ophthalmology,The Seventh Affiliated Hospital,Sun Yat-sen University,Shenzhen 518107,China;Shenzhen Research Institute,The Hong Kong Polytechnic University,Shenzhen 518052,China;Centre for Myopia Research,School of Optometry,The Hong Kong Polytechnic University,HongKong 999077,China)

机构地区：[1]中山大学附属第七医院眼科,广东深圳518107 [2]香港理工大学深圳研究院,广东深圳518052 [3]香港理工大学眼科及视光学系近视研究中心,中国香港红磡999077

出　　处：《中山大学学报(医学科学版)》2025年第2期284-292,共9页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：深圳市科技创新委员会资助项目(JCYJ20190809175801663)。

摘　　要：【目的】探讨蓝光对豚鼠眼球正视化过程的影响和蓝光在正视化过程中的潜在机制,并评估其在近视防控中的潜在应用价值。【方法】将20只3周龄雄性豚鼠随机分到白光组和蓝光组,每2周测量屈光度和眼部生物学参数,直至第8周实验结束。并利用蛋白组学技术4D-数据非依赖型采集(4D-DIA)对蓝光组和白光组的豚鼠视网膜进行检测,探索蛋白组成、表达差异以及对应的生物学功能。【结果】2周后,白光组中豚鼠逐渐向正视状态发展,蓝光组出现远视漂移,组间屈光度存在显著差异(P<0.001)。自第4周起,蓝光组的眼轴长度显著小于白光组(P<0.05)。此外,自第2周起,蓝光组的玻璃体腔长度显著小于白光组(P<0.05)。应用蛋白组学技术在视网膜中共筛选出161个差异蛋白,其中98个蛋白上调,63个蛋白下调,主要富集于囊泡运输、氧化-还原反应、烟酸及烟酰胺代谢、烟酰胺腺嘌呤二核苷酸(NAD)+代谢等生物合成途径中。【结论】暴露于蓝光的豚鼠表现出远视漂移和眼轴伸长减缓,对正视化有延缓作用。利用4D-DIA技术发现,蓝光组与白光组间差异蛋白主要参与NAD+代谢、烟酸及烟酰胺代谢途径,其中参与NAD+补救合成途径的烟酰胺磷酸核糖转移酶(NAMPT)上调,沉默信息调节因子2(SIRT2)下调。这为理解蓝光在正视化中的作用机制提供了新见解,并为近视防控技术开发提供了理论依据。【Objective】To investigate the effect of blue light on emmetropization in guinea pigs,explore the potential mechanisms and assess its application in myopia prevention and control.【Methods】Three-week-old male guinea pigs(n=20)were randomly assigned to the white light group and the blue light group.Refraction and ocular biological parameters were measured every 2 weeks until the experiment ended at week 8.And the 4D-data-independent acquisition(4D-DIA)proteomics technology was used to analyze retina from both the blue light and white light groups,exploring protein composition,expression differences,and biological functions.【Results】After 2 weeks,Guinea pigs exposed to white light gradually tended towards emmetropia,showing a statistically significant difference in refractive error compared to the blue light group(P<0.001).From week 4,the axial length of the blue light group was significantly shorter than that of the white light group(P<0.05).Meanwhile,the vitreous chamber length in the blue light group was significantly smaller than that of the white light group from week 2(P<0.05).A total of 161 differentially expressed proteins were identified by proteomics technology in the retina,with 98 proteins upregulated and 63 proteins downregulated.These proteins were primarily enriched in biosynthetic pathways such as vesicle transport,redox reaction,niacin and nicotinamide metabolism and NAD+metabolism.【Conclusions】Guinea pigs raised under blue light exhibit hyperopic drift and slowed axial elongation,which slows the procession of emmetropization.Based on the 4 D-DIA technology,the differentially expressed proteins between the blue light and white light groups are primarily involved in NAD+metabolism,niacin and nicotinamide metabolism.Especially in NAD+salvage synthesis,nicotinamide phosphoribosyl transferase(NAMPT)is upregulated,while sirtuin 2(SIRT 2)is downregulated.It provides new insights into the mechanism of blue light in emmetropization and a theoretical basis for myopia prevention and control.

关 键 词：蓝光 正视化 近视 屈光发育 蛋白组学 

分 类 号：R778[医药卫生—眼科]