Chlorogenic acid alleviates IPEC‑J2 pyroptosis induced by deoxynivalenol by inhibiting activation of the NF‑κB/NLRP3/ caspase‑1 pathway  

作　　者：Yanmei Xue Fuchang Li Rui Li Xinru Zhang Huijun Guo Chunyang Wang 

机构地区：[1]Shandong Provincial Key Laboratory of Zoonoses,College of Animal Veterinary Medicine,Shandong Agricultural University,Tai’an,Shandong 271018,China [2]College of Animal Science and Technology,Shandong Agricultural University,Tai’an,Shandong 271018,China

出　　处：《Journal of Animal Science and Biotechnology》2025年第2期797-812,共16页畜牧与生物技术杂志(英文版)

基　　金：supported by the National Natural Science Foundation of China(32373062);the Natural Science Foundation of Shandong Province(ZR2023MC144);Funds of Shandong Province Modern Agricultural Technology System Innovation Team Program(SDAIT-21-10).

摘　　要：Background Deoxynivalenol(DON)is a mycotoxin that severely pollutes feed ingredients,and methods for reducing DON toxicity have become a significant research direction.Chlorogenic acid(CGA)is an active polyphenol found in some plants,which has anti-inflammatory and antioxidant properties and a protective effect on animal intestinal health.The effects of CGA on DON-induced pyroptosis in the intestinal porcine epithelial cell line-J2(IPEC-J2)and its potential mechanism were explored in this study.Results IPEC-J2 cells viability and membrane integrity were inversely correlated with DON concentration.Compared to those in the group treated with DON alone at 2,500 ng/mL,pretreatment with 80μmol/L CGA for 4 h significantly improved cell viability(P<0.01),and the alleviation of typical pyroptotic symptoms induced by DON were observed,including reduced cellular DNA fragmentation,decreased release of lactate dehydrogenase(LDH),normalized ROS levels,restoration of extracellularCa2+andK+contents to normal levels(P<0.01),as well as suppressed the enzyme activities of caspase-1 and caspase-4(P<0.01).Additionally,the mRNA expression levels of TNF,MDP,NOD2,TLR4,ASC and GSDMD were significantly improved(P<0.01),while both mRNA and protein expression levels of NF-κB,NLRP3,caspase-1,IL-1βand IL-18 were significantly upregulated(P<0.01)in the CGA+DON group,compare to those in the DON group.Conclusion Pretreatment with 80μmol/L CGA for 4 h effectively alleviated pyroptosis in IPEC-J2 cells induced by 2,500 ng/mL of DON through inhibiting activation of the NF-κB/NLRP3/capase-1 pathway.

关 键 词：Chlorogenic acid DEOXYNIVALENOL IPEC-J2 cells PYROPTOSIS Signaling pathway 

分 类 号：S816.7[农业科学—饲料科学]