Baicalin alleviates intestinal inflammation and microbial disturbances by regulating Th17/Treg balance and enhancing Lactobacillus colonization in piglets  

作　　者：Shunfen Zhang Chengzeng Luo Kai Li Junhong Wang Huixin Wang Ruqing Zhong Liang Chen Qiugang Ma Hongfu Zhang 

机构地区：[1]State Key Laboratory of Animal Nutrition and Feeding,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China [2]College of Animal Science and Technology,China Agricultural University,Beijing 100193,China

出　　处：《Journal of Animal Science and Biotechnology》2025年第2期866-882,共17页畜牧与生物技术杂志(英文版)

基　　金：supported by the National Natural Science Foundation of China(32102582);the Youth innovation of Chinese Academy of Agricultural Sciences(Y2023QC09);Zhejiang Province Traditional Chinese Medicine Science and technology Project(2022ZB270);the Agricultural Science and Technology Innovation Program(ASTIPIAS07,cxgc-ias-16)。

摘　　要：Background Intestinal inflammation is a common and serious health problem in piglet production,especially enteritis caused by pathogenic Escherichia coli(E.coli).This condition often leads to high mortality,slow weight gain,and significant economic losses.Results In this study,we isolated an E.coli strain,SKLAN202302,from the colon of diarrheal piglets to create an intestinal inflammation model for evaluating the protective effects of baicalin.Piglets infected with E.coli exhibited significant reductions in body weight,feed intake,small intestine length,and ileal goblet cell count(P<0.05),along with deteriorated ileal morphology.However,baicalin supplementation resulted in body weights,feed intake,and intestinal morphology similar to those of the control group.Notably,there was a significant increase in the colonization of Lactobacillus species,particularly Lactobacillus_reuteri,Lactobacillus_amylovorus,and Lactobacillus_johnii,compared to the E.coli group(P<0.05).At the metabolic and transcriptional levels,E.coli infection increased inflammatory mediators,including eicosanoids(leukotriene F4,prostaglandin F1a,leukotriene E4,thromboxane B2,prostaglandin G2,and PGH2),monosaccharides,and TCA cycle intermediates(oxoglutaric acid,glutaric acid,adipic acid,citric acid,and isocitric acid)in the ileum.It also promoted the expression of genes related to autoimmune diseases and the Th17 differentiation signaling pathway(CTLA4,IFN-ALPHA-8,IL12RB2,TRAV3,TRAV16,FOS,and VEGFA),as well as inflammatory factors.Conversely,baicalin supplementation not only counteracted these effects but also enhanced the presence of metabolites such as phospholipids[including lyso PC(P-18:1(9Z)/0:0),PC(17:0/0:0),lyso PC(16:1(9Z)/0:0),PC(18:0/0:0),lyso PC(18:0/0:0),PA(10:0/i-16:0),and PA(10:0/8:0)]and amino acids.It also regulated genes within the IL-17 signaling pathway(IL4,CCL17,CXCL10,IFNG,and CXCL2),suggesting a mechanism by which baicalin mitigates E.coli-induced intestinal and microbial disturbances.Subsequent flow cytometry analysis showed

关 键 词：BAICALIN Escherichia coli Intestinal inflammation Microbiota PIGLETS Th17/Treg balance Th17 cell 

分 类 号：S852.2[农业科学—基础兽医学] S858.28[农业科学—兽医学]