Characterization of the colostrum proteome of primiparous Holstein cows and its association with colostrum immunoglobulin G concentrations  

作　　者：Ezequias Castillo-Lopez Patrick Biber Arife Sener-Aydemir Karin Hummel Ebrahim Razzazi-Fazeli Nicole Reisinger Qendrim Zebeli Susanne Kreuzer-Redmer Thomas Hartinger 

机构地区：[1]Center for Animal Nutrition and Welfare,University of Veterinary Medicine Vienna,Veterinarplatz 1,1210 Vienna,Austria [2]Christian Doppler Laboratory for Innovative Gut Health Concepts of Livestock,Vienna,Austria [3]University of Veterinary Medicine Vienna,VetCore Facility(Mass Spectrometry),Vienna,Austria [4]Dsm-Firmenich,Animal Nutrition&Health R&D Center,Tulln,Austria

出　　处：《Journal of Animal Science and Biotechnology》2025年第2期931-947,共17页畜牧与生物技术杂志(英文版)

基　　金：supported by Austrian Federal Ministry for Digital and Economic Affairs;the National Foundation for Research,Technology and Development;supported using resources of the Vet Core Facility(Mass Spectrometry)of the University of Veterinary Medicine Vienna。

摘　　要：Background The objective was to characterize the colostrum proteome of primiparous Holstein cows in association with immunoglobulin G(IgG)content.Immediately after calving,colostrum samples were collected from 18 cows to measure IgG concentration.Based on colostrum IgG content,samples were classified through cluster analysis and were identified as poor,average,and excellent quality.The proteome was assessed with quantitative shotgun proteomics;abundance data were compared among the colostrum types;enrichment analysis of metabolic processes and proteins classes was performed as well.We also tested correlations between this proteome and blood globulin level of cows and passive immunity level of calves.Results On average,428 proteins were identified per sample,which belonged mainly to cellular process,biological regulation,response to stimulus,metabolic process,and immune system process.Most abundant proteins were complement C3(Q2UVX4),alpha-S1-casein(P02662),Ig-like domain-containing protein(A0A3Q1M032),albumin(A0A140T897),polymeric immunoglobulin receptor(P81265),lactotransferrrin(P24627),and IGHG1*01(X167014).Colostrum of excellent quality had greater(P<0.05)abundance of serpin A3-7(A2I7N3),complement factorl(A0A3Q1 MIF4),lipocalin/cytosolic fatty-acid binding domain-containing protein(A0A3Q1 MRQ2),complement C3(E1B805),complement component 4 binding protein alpha(A0AAF6ZHP5),and complement component C6(F1MM86).However,colostrum of excellent quality had lower(P<0.05)abundance of HGF activator(E1BCW0),alpha-S1-casein(P02662),and xanthine dehydrogenase/oxidase(P80457).This resulted in enrichment of the biological processes predominantly for complement activation alternative pathway,complement activation,complement activation classical pathway,humoral immune response,leukocyte mediated immunity,and negative regulation of endopeptidase activity in excellent-quality colostrum.Additionally,some colostrum proteins were found to be correlated with the blood globulin level of cows and with the passive immunity level of ca

关 键 词：Bovine colostrum COW IMMUNOGLOBULINS Passive immunity PROTEOMICS 

分 类 号：S823[农业科学—畜牧学]