基于HS-GC-IMS的不同部位及成熟度烟叶挥发性成分差异分析  

Differential analysis of volatile components in tobacco leaves of varying parts and maturity based on HS-GC-IMS

作　　者：张亮 刘显军 孙海峰 陈壮壮 何澎 杨鹏飞 吴薇 尚紫博 ZHANG Liang;LIU Xianjun;SUN Haifeng;CHEN Zhuangzhuang;HE Peng;YANG Pengfei;WU Wei;SHANG Zibo(Technology R&D Center,Shenzhen Tobacco Industrial Co.,Ltd.,Shenzhen 518000,China;College of Tobacco Science and Engineering,Zhengzhou University of Light Industry,Zhengzhou 450001,China)

机构地区：[1]深圳烟草工业有限责任公司技术研发中心,广东深圳518000 [2]郑州轻工业大学烟草科学与工程学院,河南郑州450001

出　　处：《轻工学报》2025年第2期116-126,共11页Journal of Light Industry

基　　金：中国烟草总公司重大科技项目(110202201053(SJ-03));中国烟草实业发展中心科技项目(ZYSYQ-2022-17)。

摘　　要：为研究湖南永州烟叶挥发性成分的差异,采用顶空-气相色谱-离子迁移谱(HS-GC-IMS)技术对不同部位及成熟度烟叶中挥发性成分进行检测,并结合主成分分析、层次聚类分析和偏最小二乘法回归分析等对比烟叶间挥发性成分含量的差异。结果表明:从不同部位及成熟度烟叶样品中共鉴定出69种挥发性成分;不同部位及成熟度烟叶样品中挥发性成分的含量均存在较明显的差异,且存在明显区域归属;共筛选出26种差异挥发性成分,其中,2-己酮、甲酸异戊酯、糠醛、2,6-二甲基吡嗪、2-甲氧基-3-异丁基吡嗪、乙酸乙酯和香叶醇等成分在不同部位烟叶中的相对含量差异较大,而四氢噻吩,2-己酮、甲酸异戊酯、壬醛、乙酸、吲哚、水杨醛和异丁醇等成分在不同成熟度烟叶中的相对含量差异较大,这些物质可用于区分不同部位烟叶和成熟度烟叶。To investigate the differences in volatile components of tobacco leaves from Yongzhou,Hunan,headspace-gas chromatography-ion mobility spectrometry(HS-GC-IMS)was employed to analyze volatile components in tobacco leaves with various parts and maturity levels.Differences of relative content were evaluated using principal component analysis,hierarchical cluster analysis,and orthogonal partial least squares regression analysis.The results showed a total of 69 volatile components were identified from tobacco leaves of different parts and maturity levels.Additionally,noticeable variations were observed in the volatile components among different tobacco leaves,with clear regional affiliations.The total 2b distinct volatile components were identified.Among these,the relative concentrations of 2-hexanone,isoamyl formate,furfural,2,6-dimethylpyrazine,2-methoxy-3-isobutylpyrazine,ethyl acetate,and geraniol exhibited significant variation in tobacco leaves depending on their stark positions within the plant.Concurrently,the relative concentrations of tetrahydrothiophene,2-hexanone,isoamyl formate,nonanal,acetic acid,indole,salicylaldehyde,and isobutanol showed significant variation in tobacco leaves at different stages of maturity.These findings could be used to distinguish tobacco leaves from different parts and maturity stages.

关 键 词：顶空-气相色谱-离子迁移谱 烟叶部位 烟叶成熟度 挥发性成分 

分 类 号：TS411[农业科学—烟草工业]