α-酮戊二酸对高脂日粮下断奶仔猪氨基酸代谢及肠粘膜形态的影响研究  

Effects ofα-ketoglutarate on Amino Acid Metabolism and Intestinal Mucosal Morphology of Weaned Piglets under High-fat Diet

作　　者：郑学斌[1] 肖瑀 许睿 ZHENG Xue-bin;XIAO Yu;XU Rui(School of Biological Engineering,Hefei Vocational and Technical College,Hefei 238000,Anhui)

机构地区：[1]合肥职业技术学院生物工程学院,安徽合肥238000

出　　处：《陇东学院学报》2025年第2期89-95,共7页Journal of Longdong University

基　　金：安徽省高校自然科学研究资助项目“乳猪益生营养无抗饲料配制的关键技术研究”(KJ2021A1391);安徽省质量工程项目“合肥职业技术学院安徽中青检验检测有限公司食品加工示范实验实训中心”(2020sxzx44)。

摘　　要：为了观察不同剂量α-酮戊二酸(AKG)对断奶仔猪组织中氨基酸代谢及肠粘膜形态学的影响,选取断奶仔猪40只,其中对照组10只,用高脂饲料喂养;试验I组(0.5%AKG+高脂日粮)、试验Ⅱ组(1%AKG+高脂日粮)、试验Ⅲ组(1.5%AKG+高脂日粮)。饲养期间自由采食,连续喂养8周后,采集小肠、盲肠、结肠、血清、肝脏及腿部肌肉,检测肠道黏膜形态及氨基酸成分。结果显示:AKG组空肠、盲肠和结肠的绒毛高度均显著高于对照组(p<0.05),且空肠、回肠和结肠的黏膜厚度也显著性增厚(p<0.05);与对照组比较,AKG的添加显著提升血清中部分生糖氨基酸、支链氨基酸Ile和芳香氨基酸Trp含量(p<0.05);而肝脏中,AKG显著降低部分生糖氨基酸和芳香氨基酸含量(p<0.05);腿肌中,AKG添加极显著降低支链氨基酸及芳香氨基酸含量(p<0.05)。AKG的添加通过降低肝脏和肌肉中生糖氨基酸含量,延缓肥胖模型断奶仔猪体重的增加,并对高脂饮食喂养的断奶仔猪肠黏膜形态具有保护作用。To observe the effects of different doses ofα-ketoglutarate(AKG)on amino acid metabolism and intestinal mucosal morphology in weaned piglets.40 weaned piglets,including 10 in the control group,were fed with high-fat diet;test group I(0.5%AKG+high-fat diet),test group II(1%AKG+high-fat diet),test group III(1.5%AKG+high-fat diet)%AKG+high-fat diet.During the feeding period,the animals were fed ad libitum,and after 8 weeks of continuous feeding,the small intestine,cecum,colon,serum,liver and leg muscles were collected to detect the intestinal mucosal morphology and amino acid composition.The results showed that the villi heights of the jejunum,cecum and colon in the AKG group were significantly higher than those in the control group(p<0.05),and the mucosal thicknesses of the jejunum,ileum and colon were thicker(p<0.05).The addition of AKG significantly increased the contents of some glycogenic amino acids,branched-chain amino acids Ile and aromatic amino acids Trp in serum(p<0.05);while in the liver,AKG significantly decreased the contents of some glycogenic amino acids and aromatic amino acids(p<0.05);Among them,the addition of AKG significantly reduced the content of branched-chain amino acids and aromatic amino acids(p<0.05).AKG supplementation delayed the weight gain of weaned piglets in the obese model by reducing the content of glycogenic amino acids in liver and muscle,and had a protective effect on the intestinal mucosal morphology of weaned piglets fed a high-fat diet.

关 键 词：高脂饮食 断奶仔猪 肠黏膜 氨基酸代谢 

分 类 号：TS251[轻工技术与工程—农产品加工及贮藏工程]