兔源解淀粉芽孢杆菌的分离鉴定及益生特性研究  

Study of isolation,identification and probiotic characteristic of rabbit-derived Bacillus amyloliquefaciens strain

作　　者：邢芳芳 韩丽 葛祥军 周振见 厉建兵 宋吉青 XING Fangfang;HAN Li;GE Xiangjun;ZHOU Zhenjian;LI Jianbing;SONG Jiqing

机构地区：[1]临沂市河东区畜牧发展促进中心,山东临沂276799 [2]金正大生态工程集团股份有限公司,山东临沂276700

出　　处：《饲料研究》2025年第4期87-91,共5页Feed Research

基　　金：2023年度泰山产业领军人才工程项目。

摘　　要：试验旨在筛选兔源菌种,研究和开发兔用微生态制剂。选取健康肉兔的新鲜粪便为试验原料,从中筛分出1株芽孢杆菌,对其进行形态特征观察、生理生化鉴定、分子生物学鉴定,以及耐酸碱、耐高温、益生特性等方面的研究。结果显示,经形态特征观察、生理生化鉴定、分子生物学鉴定确定分离株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),命名为XR01。XR01在pH值2.0的条件下存活率可达84.25%,在胆碱浓度0.4%的条件下存活率可达到42.30%,在100℃处理后存活率仍可达到91.80%,且对大肠埃希氏菌和铜绿假单胞菌具有一定的抑制作用,小白鼠喂服安全。研究表明,该解淀粉芽孢杆菌菌株安全,易于发酵培养,且具有良好的耐高温、耐酸、胆碱等益生特性,可作为兔用微生态制剂的候选菌株。The experiment was aimed at screening rabbit-derived strains and developing microecological preparations for rabbits.Fresh feces from healthy meat rabbits were selected as the experimental material,and one strain of Bacillus was screened out from it.The morphological characteristics,physiological and biochemical identification,molecular biological identification,as well as the acid and alkali resistance,high temperature resistance,and probiotic characteristics of the strain were studied.The results showed that the isolate was identified as a strain of Bacillusamyloliquefaciens through morphological observation,physiological and biochemical identification,and molecular biological identification,and the isolate was named XR01.The isolate XR01 had a survival rate of 84.25%under pH value 2.0 conditions,a survival rate of 42.30%under choline concentration of 0.4%,and a survival rate of 91.80%after treatment at 100℃.It also had certain inhibitory effects on Escherichiacoliand Pseudomonasaeruginosa,and was safe for mice to consume.The study showed that the Bacillusamyloliquefaciens strain was safe,easy to ferment and cultivate,and has good probiotic characteristics such as high temperature resistance,acid resistance,and choline resistance,and can be used as a candidate strain for rabbit microecological preparations.

关 键 词：微生态制剂 益生特性 分离鉴定 解淀粉芽孢杆菌 兔源 

分 类 号：S816.7[农业科学—饲料科学]