红花籽粕中2-羟基牛蒡子苷和罗汉松树脂苷的提取及高效液相检测方法的建立与应用研究  

Study on extraction and establishment of a HPLC detection method and application of 2-hydroxyarctiin and matairesinoside in safflower seed cake

作　　者：王雪 王甜[1,2] 胡建勇 李林[1,2] 李晓东 王丽[1,2] 高攀 WANG Xue;WANG Tian;HU Jianyong;LI Lin;LI Xiaodong;WANG Li;GAO Pan

机构地区：[1]新疆维吾尔自治区水产科学研究所,新疆乌鲁木齐830000 [2]农业农村部西北地区渔业资源环境科学观测实验站,新疆乌鲁木齐830000

出　　处：《饲料研究》2025年第4期119-123,共5页Feed Research

基　　金：新疆维吾尔自治区自然科学基金面上项目(项目编号:2022D01A122、2022D01A123);现代农业产业技术体系(项目编号:CARS-45)。

摘　　要：试验旨在通过建立红花籽粕中2-羟基牛蒡子苷和罗汉松树脂苷的提取和检测方法,探索鱼产品饲料中红花籽粕的适宜添加量。采用80%乙醇超声提取红花籽粕中2-羟基牛蒡子苷和罗汉松树脂苷,并利用高效液相色谱(HPLC)紫外检测器进行测定。结果表明,2-羟基牛蒡子苷标准液的线性回归方程为:y=10.06885x+0.59591,在2.5~50.0 mg/L范围内线性关系良好,相关系数大于0.9999;在0.5 mg/L添加水平下,平均回收率为94.86%~104.69%,检测限为0.03 mg/L,定量限为0.10 mg/L。罗汉松树脂苷标准液的线性回归方程为:y=15.79781x-17.84546,在2.5~20.0 mg/L范围内线性关系良好,相关系数大于0.9951;在0.5 mg/L添加水平下,平均回收率为99.31%~105.16%,检测限为0.12 mg/L,定量限为0.40 mg/L。研究表明,试验所建立的高效液相色谱法操作简便、准确、重复性好,可应用于红花籽粕中2-羟基牛蒡子苷和罗汉松树脂苷的含量测定。The experiment aims to establish extraction and detection methods for 2-hydroxyarctiin and matairesinoside in safflower seed cake to explore the appropriate addition level of safflower seed cake in fish product feed.The extraction of 2-hydroxy-arctioside and podocarpinus resinoside from safflower seed cake by ultrasonography with 80%ethanol was studied and determined by high performance liquid chromatography(HPLC)ultraviolet detector.The results showed that the linear regression equation for 2-hydroxyarctiin standard solution was y=10.06885x+0.59591.It exhibited a good linear relationship in the range of 2.5~50.0 mg/L,with a correlation coefficient greater than 0.9999.At the addition level of 0.5 mg/L,the average recovery rate was 94.86%~104.69%,with a detection limit of 0.03 mg/L and a quantification limit of 0.10 mg/L.The linear regression equation for matairesinoside standard solution was y=15.79781x-17.84546.It exhibited a good linear relationship in the range of 2.5~20.0 mg/L,with a correlation coefficient greater than 0.9951.At the addition level of 0.5 mg/L,the average recovery rate was 99.31%~105.16%,with a detection limit of 0.12 mg/L and a quantification limit of 0.40 mg/L.The study indicates that the HPLC method established in the experiment is simple,accurate,and has good repeatability.It can be applied to the determination of the content of 2-hydroxyarctiin and matairesinoside in safflower seed cake.

关 键 词：红花籽粕 2-羟基牛蒡子苷 罗汉松树脂苷 检测方法 

分 类 号：S816.17[农业科学—饲料科学]