沙棘混合油对小鼠慢性酒精肝损伤及肠道菌群的调节作用  

Regulatory effects of sea buckthorn mixed oil on chronic alcohol-induced liver injury and intestinal microbiota in mice

作　　者：王芳[1,2] 张滨 张鑫 赵普润 李博文 霍乃蕊 祁曣槿[1] 秦旭巍 郭建峰 Wang Fang;Zhang Bin;Zhang xin;Zhao Purun;Li Bowen;Huo Nairui;Qi Yanjin;Qin Xuwei;Guo Jianfeng(College of Chemistry and Chemical Engineering,North University of China,Taiyuan 030051,China;Shanxi Provincial Key Laboratory for Chinese Lujiu Plant Extraction and Health,Fenyang 030051,China;International Seabuckthorn Association,Beijing,100038,China;College of biological engineering,University of Science and Technology,Tianjin,300222,China;College of Veterinary Medicine,Shanxi Agricultural University,J inzhong 030801,China)

机构地区：[1]中北大学化学与化工学院,山西太原030051 [2]中国露酒植物提取与健康山西省重点实验室,山西汾阳032200 [3]国际沙棘协会,北京100038 [4]天津科技大学生物工程学院,天津300222 [5]山西农业大学动物医学学院,山西晋中030801

出　　处：《山西农业大学学报(自然科学版)》2025年第2期39-49,共11页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：吕梁市重点研发项目(2023SHFZ03);吕梁市校地合作重点研发专项(2023XDHZ06)。

摘　　要：[目的]本文旨在研究特定脂肪酸比例的沙棘混合油对小鼠慢性酒精肝损伤和肠道菌群的影响。[方法]沙棘果油和沙棘籽油按一定比例配制成沙棘混合油,其中饱和脂肪酸(SFA):单不饱和脂肪酸(MUFA):多不饱和脂肪酸(PUFA)比例为1∶1.41∶1.48。小鼠随机分为空白对照(NC)组、乙醇模型(AC)组、混合油高剂量干预(HM)组(750 mg/kg bw)、混合油中剂量干预(MM)组(600 mg/kg bw)和混合油低剂量干预(LM)组(450 mg/kg bw),采用递增乙醇浓度灌胃方式造模6周后,测定肝功能、氧化应激及炎症因子等18个生化指标;苏木精-伊红染色观察肝组织病理学改变;取小鼠粪便进行16S rRNA测序,分析肠道菌群多样性。[结果]与AC组相比,三个剂量沙棘混合油干预均能够显著改善慢性酒精摄入引起的肝脏脂肪变性,降低肝功能标志物谷草转氨酶(AST)、谷丙转氨酶(ALT)水平,降低肝总甘油三酯(TG)、总胆固醇(TC)和低密度脂蛋白(LDL)水平及肝指数水平,降低肝丙二醛(MDA)、过氧化物酶(MPO)等氧化应激标志物及白介素⁃1(IL⁃1)、白介素⁃6(IL⁃6)和肿瘤坏死因子⁃α(TNF⁃α)等炎症因子水平,降低了肝脏和粪便中总胆汁酸(TBA)水平;提高了肝脏高密度脂蛋白(HDL)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)水平。其中MM组效果最好,HM组次之。肠道微生物组分析表明,慢性酒精暴露能降低小鼠肠道微生物β多样性,改变微生物组成与功能。沙棘混合油干预增加了菌群β多样性,在科水平上提高了Akkermansia、Ruminococcaceae等菌群丰度,降低了志贺氏菌病等功能丰度。[结论]SFA∶MUFA∶PUFA比例为1∶1.41∶1.48的沙棘混合油可以通过减缓抗氧化水平、炎症因子以及调节调节小鼠肠道菌群等多种方式有效缓解小鼠慢性酒精肝损伤。[Objective]The current study was aimed to investigate the effects of sea-buckthorn mixed oil with a specific fatty acid ratio on chronic alcohol-induced liver injury and intestinal microbiota in mice.[Methods]Sea-buckthorn fruit oil and seed oil were mixed in a specific ratio to prepare sea-buckthorn oil mixtures with a saturated fatty acid(SFA):monounsaturated fatty acid(MUFA):polyunsaturated fatty acid(PUFA)ratio of 1∶1.41∶1.48.Mice were randomly divided into a normal control(NC)group,an ethanol model(AC)group,a high-dose mixed oil intervention(HM)group(750 mg/kg bw),a medium-dose mixed oil(MM)group(600 mg/kg bw),and a low-dose mixed oil group(LM)(450 mg/kg bw).A chronic alcoholic liver in-jury(ALD)model was established by increasing ethanol concentration consumption in mice.After six weeks of modeling by in-cremental ethanol gavage,18 biochemical indicators,including liver function,oxidative stress,and inflammatory factors,were measured.Hematoxylin-eosin(HE)staining was used to observe hepatic histopathological changes.Mouse feces were collect-ed for 16S rRNA sequencing to analyze intestinal microbiota diversity.[Results]Compared with the AC group,all three doses of sea buckthorn mixed oil intervention significantly alleviated chronic alcohol-induced hepatic steatosis,reduced liver function markers such as alanine transaminase(ALT)and aspartate transaminase(AST),reduced hepatic total triglyceride(TG),to-tal cholesterol(TC),low-density lipoprotein(LDL),and liver index levels,and lowered oxidative stress markers such as malondialdehyde(MDA)and peroxidase(MPO),as well as inflammatory factors such as interleukin-1(IL-1),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α).The intervention also reduced total bile acid(TBA)levels in the liver and fe-ces,while increasing high-density lipoprotein(HDL),superoxide dismutase(SOD)and glutathione(GSH)levels in the liver.The medium-dose intervention(MM)group exhibited the best effects,followed by the HM group.Intestinal microbiota analy-sis indicated that chronic al

关 键 词：特定饱和度脂肪酸配制 沙棘混合油 慢性酒精肝损伤 肠道菌群 胆酸 

分 类 号：TS201.4[轻工技术与工程—食品科学] R151.1[轻工技术与工程—食品科学与工程]