新城疫病毒HN蛋白的截短表达及间接ELISA方法建立  

Truncated Expression of HN Protein of Newcastle Disease Virus and Establishment of Indirect ELISA Method

作　　者：魏玲[1] 王思怡[1] 熊荣园 梁洪 王怀禹[1] 李彩虹[1] 杨国淋 WEI Ling;WANG Siyi;XIONG Rongyuan;LIANG Hong;WANG Huaiyu;LI Caihong;YANG Guolin(Nanchong Vocational and Technical College,Nanchong,Sichuan 637131;North Sichuan Medical College,Nanchong,Sichuan 637131,China)

机构地区：[1]南充职业技术学院,四川南充637131 [2]川北医学院,四川南充637100

出　　处：《家畜生态学报》2025年第3期91-95,共5页Journal of Domestic Animal Ecology

基　　金：南充市科技计划项目(22YYJCYJ0041)。

摘　　要：为了建立一种快速的新城疫病毒(Newcastle disease virus,NDV)抗体检测方法,试验采用原核表达技术表达HN蛋白,以HN蛋白为检测抗原建立酶联免疫吸附测定(Enzyme linked immunosorbent assay,ELISA)方法。结果表明,获得了以包涵体形式表达的重组蛋白,间接ELISA方法检测6种其它鸡病阳性血清均为阴性,其最低抗体检出效价为1∶10240,其与血凝抑制试验(Hemagglutination inhibition,HI)方法的阳性和阴性符合率分别为98.08%和92.31%,检测临床样品阳性率为91.48%。说明建立的间接ELISA方法可用于NDV免疫抗体检测和疫苗免疫效果评价。To establish a rapid method for detecting antibodies against Newcastle disease virus(NDV),the HN protein was truncated and expressed by prokaryotic expression technology,and the NDV enzyme linked immunosorbent assay(ELISA)method was established with the expressed recombinant protein.The results showed that the target protein was expressed in the form of inclusion body.Indirect ELISA method was employed to detect the positive sera of six other chicken diseases,and the results were all negative,with the lowest antibody detection titer of 1:10240.The positive and negative coincidence rate of which with Hemagglutination inhibition test(HI)method were 98.08%and 92.31%,respectively.The positive rate of clinical samples was 91.48%.It indicates that indirect ELISA method can be used for NDV immune antibody detection and vaccine immune efficacy evaluation.

关 键 词：新城疫病毒 HN蛋白 截短表达 间接ELISA 抗体检测 

分 类 号：S858.31[农业科学—临床兽医学]