丝状真菌全局性调控因子LaeA的生物信息学分析  

Bioinformatics Analysis of Global Regulatory Factor LaeA in Filamentous Fungi

作　　者：刘惠怡 刘主[1] 陈庆泽 许喜佳 陈凯 康林芝 LIU Huiyi;LIU Zhu;CHEN Qingze;XU Xijia;CHEN Kai;KANG Linzhi(School of Biology and Agriculture,Shaoguan University,Shaoguan Guangdong 512005;School of Food Science&Technology,Shaoguan University,Shaoguan Guangdong 512005;Shaoguan Xinghe Biotechnology Co.,Ltd.,Shaoguan Guangdong 512005)

机构地区：[1]韶关学院生物与农业学院,广东韶关512005 [2]韶关学院食品学院,广东韶关512005 [3]韶关市星河生物科技有限公司,广东韶关512005

出　　处：《现代农业科技》2025年第7期145-149,共5页Modern Agricultural Science and Technology

基　　金：广东省韶关学院大学生创新创业训练计划项目(S202210576063);广东省韶关学院科研重点项目(SZ2018KJ07);广东省普通高校青年创新人才类项目(2021KQNCX086);广东省教育厅普通高校重点项目(2022ZDZX2058)。

摘　　要：通过应用生物信息学分析方法,对丝状真菌中的全局性调控因子LaeA进行结构与功能预测,旨在为揭示其在丝状真菌次级代谢过程中的作用提供科学依据。结果表明:丝状真菌全局性调控因子LaeA是亲水蛋白,无跨膜区且无信号肽;在氨基酸序列第250~275位之间可能存在卷曲结构;二级结构中,α-螺旋所占比例为29.84%,β-折叠占15.59%,β-转角占4.30%,无规则卷曲占50.27%;在第138~226位之间有1个结构域Methyltransf_25 domain;预测结果显示,该蛋白氨基酸序列不能够得到较为理想的蛋白质三维结构模型;LaeA蛋白具有翻译途径tRNA甲基化酶以及Ⅰ类SAM依赖性甲基转移酶的作用。By applying bioinformatics analysis methods,the structure and function of the global regulatory factor LaeA in filamentous fungi were predicted,so as to provide a scientific basis for revealing its role in the secondary metabolism of filamentous fungi.The results showed that the global regulatory factor LaeA in filamentous fungi was a hydrophilic protein with no transmembrane region or signal peptide.A coiled structure might exist between amino acid residues 250-275.In the secondary structure,α-helices accounted for 29.84%,β-pleated sheets accounted for 15.59%,β-turns accounted for 4.30%,and random coils accounted for 50.27%.A domain(Methyltransf_25 domain)was identified between residues 138-226.The prediction results indicated that the amino acid sequence of this protein could not yield an ideal three-dimensional structural model.The LaeA protein functioned as a tRNA methyltransferase and a classⅠSAM-dependent methyltransferase.

关 键 词：丝状真菌 全局性调控因子 LAEA 生物信息学 疏水性 结构域 

分 类 号：Q933[生物学—微生物学]