赤芍总苷抑制甲状腺癌细胞恶性行为的机制研究  

Mechanism of total peony glycosides for inhibiting malignant behaviors of thyroid cancer cells

作　　者：曹马狄 王科[2] 杨爱民[3] Cao Madi;Wang Ke;Yang Aimin(Department of General Surgery,Xi'an Workers Hospital,Xi'an 710000,China;Department of Otorhinolaryngology,Head,and Neck Surgery,First Hospital,Xi'an Medical University,Xi'an 710000,China;Department of Nuclear Medicine,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710000,China)

机构地区：[1]西安工会医院普外科,西安710000 [2]西安医学院第一附属医院耳鼻喉头颈外科,西安710000 [3]西安交通大学第一附属医院核医学科,西安710000

出　　处：《国际医药卫生导报》2025年第8期1275-1282,共8页International Medicine and Health Guidance News

基　　金：国家自然科学基金(81871389)。

摘　　要：目的探讨赤芍总苷(TPG)对甲状腺癌细胞(SW579)恶性行为的影响和机制。方法收集2016年6月至2019年12月西安工会医院收治的90例甲状腺癌患者癌组织及癌旁组织,实时荧光定量逆转录PCR(RT-qPCR)检测甲状腺癌组织中circ_HOMER1表达水平,并探讨circ_HOMER1表达与甲状腺癌患者临床病理特征的关系。将SW579细胞分为对照(con)组、TPG 50 mg/L组、TPG 100 mg/L组、TPG 200 mg/L组、si-NC组、si-circ_HOMER1组、TPG+anti-miR-23a-3p组、TPG+pcDNA-circ_HOMER1组。RT-qPCR检测circ_HOMER1和miR-23a-3p表达,Transwell法检测细胞迁移和侵袭,平板克隆实验和CCK-8法检测细胞增殖。circ_HOMER1和miR-23a-3p的靶向关系通过双荧光素酶报告实验确定。评估circ_HOMER1表达与患者临床病理特征的相关性。统计学方法采用χ^(2)检验、单因素方差分析。结果甲状腺癌组织中circ_HOMER1表达上调。circ_HOMER1表达与TNM分期、淋巴结转移、包膜浸润均相关(均P<0.05)。TPG 50 mg/L组、TPG 100 mg/L组、TPG 200 mg/L组circ_HOMER1水平均低于对照组(0.78±0.04、0.47±0.03、0.23±0.02比1.00±0),miR-23a-3p水平均高于对照组(1.49±0.03、2.11±0.06、2.89±0.09比1.00±0),差异均有统计学意义(F=1425.931、1904.071,均P<0.05);迁移数、克隆数、侵袭数均低于对照组,细胞抑制率高于对照组(均P<0.05)。si-circ_HOMER1组SW579细胞迁移、增殖和侵袭行为均低于si-NC组(均P<0.05);TPG+pcDNA-circ_HOMER1组、TPG+anti-miR-23a-3p组增殖、迁移和侵袭行为均高于TPG组(均P<0.05)。miR-23a-3p是circ_HOMER1的靶基因。结论TPG可能通过下调circ_HOMER1/miR-23a-3p轴抑制甲状腺癌细胞恶性增殖、迁移和侵袭行为。Objective To investigate the effect and mechanism of total peony glycosides(TPG)on the malignant behaviors of thyroid cancer cells(SW579).Methods The cancer tissue and their adjacent tissue of 90 patients with thyroid cancer who were diagnosed and received surgical treatment in Xi'an Workers Hospital from June 2016 to December 2019 were collected.The circ_HOMER1 expression level in the thyroid cancer tissue was detected by the real-time fluorescent quantitative reverse transcription PCR(RT-qPCR);the relationship between circ_HOMER1 expression and clinicopathological characteristics of the patients was explored.The SW579 cells were divided into a control(con)group,a TPG 50 mg/L group,a TPG 100 mg/L group,a TPG 200 mg/L group,an si-NC group,an si-circ_HOMER1 group,a TPG+anti-miR-23a-3p group,and a TPG+pcDNA-circ_HOMER1 group.The RT-qPCR was performed to detect the expressions of circ_HOMER1 and miR-23a-3p;the Transwell method was employed to detect the cell migration and invasion;the plate cloning and CCK-8 assays were used to detect the cell proliferation.The targeting relationship between circ_HOMER1 and miR-23a-3p was determined by the dual luciferase reporter assay.The correlation between circ_HOMER1 expression and clinicopathological features was evaluated.χ^(2) test and one-way analysis of variance were used for the statistical analysis.Results The expression of circ_HOMER1 was up-regulated in the thyroid cancer tissue.The circ_HOMER1 expression was correlated with TNM stage,lymph node metastasis,and envelope infiltration(all P<0.05).The expression levels of circ_HOMER1 in the TPG 50 mg/L group,the TPG 100 mg/L group,and the TPG 200 mg/L group were lower than that in the control group(0.78±0.04,0.47±0.03,and 0.23±0.02 vs.1.00±0),while the levels of miR-23a-3p were higher(1.49±0.03,2.11±0.06,and 2.89±0.09 vs.1.00±0),with statistical differences(F=1425.931 and 1904.071;both P<0.05).The numbers of migration,clone,and invasion in in the TPG 50 mg/L group,the TPG 100 mg/L group,and the TPG 200 mg/L group

关 键 词：甲状腺癌 赤芍总苷 circ_HOMER1 增殖 迁移 侵袭 miR-23a-3p 

分 类 号：R736.1[医药卫生—肿瘤]