LINC00641靶向miR-320b/KLF7对胃癌细胞增殖、侵袭、血管生成拟态的影响  

The Effects of LINC00641 on the Proliferation,Invasion,and Vascular Mimicry of Gastric Cancer Cells by Targeting miR-320b/KLF7

作　　者：汪家富 黄晨[2,3] 朱纯超[2,3] Wang Jiafu;Huang Chen;Zhu Chunchao(Department of General Surgery,Shanghai Minhang District Pujiang Hospital&.Minhang Branch of Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 201112,China;Department of Gastrointestinal Surgery,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 201100,China;Department of Surgical Teaching and Research,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China)

机构地区：[1]上海市闵行区浦江医院&上海交通大学医学院附属仁济医院闵行分院普外科,上海201112 [2]上海交通大学医学院附属仁济医院胃肠外科,上海每201100 [3]上海交通大学医学院外科教研室,上海手200025

出　　处：《南开大学学报(自然科学版)》2025年第1期78-84,共7页Journal of Nankai University(Natural Sience)

基　　金：上海市卫生健康委员会中医药科研项目(2022QN018)。

摘　　要：探究长链非编码RNA641(LINC00641)靶向微小核苷核酸320b(miR-320b)/Kruppel样因子7(KLF7)对胃癌细胞增殖、侵袭、血管生成拟态的影响.将SGC-7901细胞分为control组、si-NC组、si-LINC00641组、si-LINC00641+miR inhibitor-NC组、si-LINC00641+miR-320b inhibitor组.通过CCK-8、EdU染色、Transwell小室、细胞划痕、流式细胞术和三维细胞培养实验分别检测细胞增殖、迁移、侵袭、凋亡和血管生成拟态;qRT-PCR检测细胞中LINC00641、miR-320b和KLF7 mRNA水平;western blot检测KLF7、VE-cadherin、N-cadherin和E-cadherin蛋白表达;双荧光素酶实验验证LINC00641、KLF7与miR-320b间的靶向关系.结果显示:沉默LINC00641可抑制SGC-7901细胞增殖、侵袭和血管生成,下调KLF7 mRNA和KLF7、VE-cadherin、N-cadherin蛋白表达(P<0.05),促进细胞凋亡,上调miR-320b水平和E-cadherin蛋白表达(P<0.05).加入miR-320b抑制剂可逆转沉默LINC00641对SGC-7901细胞增殖、侵袭、凋亡和血管生成的影响(P<0.05),上调KLF7 mRNA和KLF7、VE-cadherin、N-cadherin蛋白表达(P<0.05),下调miR-320b水平和E-cadherin蛋白表达(P<0.05);LINC00641与miR-320b、miR-320b和KLF7均存在靶向关系.上述结果表明:沉默LINC00641可通过靶向miR-320b/KLF7轴,抑制胃癌细胞增殖、侵袭和血管生成拟态.To investigate the effects of long non-coding RNA641(LINCO0641)on the proliferation,invasion,and vascular mimicry of gastric cancer cells by targeting microRNA 320b(miR-320b)/Kruppel like factor 7(KLF7).SGC-7901 cells were assigned into control group,si-NC group,si-LINC00641 group,si-LINCO0641+miR inhibitor NC group,and si-LINC00641+miR-320b inhibitor group.CCK-8,EdU staining,transwell chamber,cell scratch assay,flow cytometry,and three-dimensional cell culture experiments were applied to detect cell proliferation,migration,invasion,apoptosis,and vascular mimicry.QRT-PCR was applied to detect the mRNA levels of LINC00641,miR-320b,and KLF7 in cells.Western blot was applied to detect the protein expression of KLF7,VE cadherin,N-cadherin,and E-cadherin.Dual luciferase assay was applied to verify the targeting relationship between LINCO0641,KLF7,and miR-320b.The results showed that silencing LINC00641 could inhibit the proliferation,invasion,and angiogenesis of SGC-7901 cells,downregulate KLF7 mRNA and KLF7,VE cadherin,and N-cadherin protein expression(P<0.05),promote cell apoptosis,and upregulate miR-320b level and E-cadherin protein expression(P<0.05).The addition of miR-320b inhibitors could reverse the effects of silencing LINCO0641 on the proliferation,invasion,apoptosis,and angiogenesis of SGC-7901 cells(P<0.05),upregulate KLF7 mRNA and KLF7,VE cadherin,N-cadherin protein expression(P<0.05),and downregulate miR-320b levels and E-cadherin protein expression(P<0.05).LINC00641 had a targeted relationship with miR-320b,and miR-320b had a targeted relationship with KLF7.The above results show that silencing LINCO0641 can inhibit proliferation,invasion,and vascular mimicry of gastric cancer cells by targeting the miR-320b/KLF7 axis.

关 键 词：长链非编码RNA641 微小核糖核酸320b Kruppel样因子7 胃癌 血管生成拟态 

分 类 号：R735.2[医药卫生—肿瘤]