基于多指标联合多元统计分析的不同花色唐古特大黄育种材料筛选  

Screening of Rheum tanguticum breeding materials with different flower colors based on multi-index combined multivariate statistical analysis

作　　者：刘东 赵强 段莹 高展 孟瑾瑾 陈红刚[1] 杜弢[1] LIU Dong;ZHAO Qiang;DUAN Ying;GAO Zhan;MENG Jinjin;CHEN Honggang;DU Tao(Gansu University of Chinese Medicine,Lanzhou,Gansu 730000,China;Qilu Institute of Technology,Jinan,Shandong 250000,China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]齐鲁理工学院,山东济南250000

出　　处：《干旱地区农业研究》2025年第2期1-12,共12页Agricultural Research in the Arid Areas

基　　金：国家中药材产业技术体系建设专项资金(CARS-21);甘肃省科技特派团专项“唐古特大黄种子丸粒化技术研究及栽培模式创新示范”(23CXNA0033)。

摘　　要：以18个不同花色唐古特大黄为研究材料,通过观察和测定根部性状及9种化合物含量,基于相关性分析、主成分分析、熵权TOPSIS法分析,对唐古特大黄育种材料进行综合质量评价,筛选表现优良的唐古特大黄育种材料。结果表明,QH003yyf(S2)、TGT(S14)、ZH002QS(S18)等材料无病害、单根质量高(0.97~1.54 kg)、须根和芦头粗(31.72~36.00 mm,58.32~68.47 mm)且长(30.5~36.1 cm,5.1~6.8 cm);18个不同花色唐古特大黄根部的大黄素、大黄酸、大黄酚、芦荟大黄素、大黄素甲醚的总含量符合药典标准要求,其中QH002yyf(S16)、DH002yyfA(S3)、HS002yyf(S1)、TGTYS(S6)、TGT(S14)、ZH001DH(S8)共6个材料的9种化合物总含量高,分别为48.86、46.41、45.88、44.96、44.23、43.81 mg·g-1;熵权TOPSIS法分析综合排序前2位的品系为QH004yyf(S7)和ZH002QS(S18)。综上,TGT(S14)和ZH002QS(S18)可作为唐古特大黄品种选育和栽培的基础材料。本研究建立的基于多指标联合多元统计分析筛选不同花色唐古特大黄育种材料的评价模式,也可用于其他育种材料的筛选评价。To identify suitable breeding materials of Rheum tanguticum,eighteen Rheum tanguticum Maxim.plants with different flower colors were selected as research materials.Comprehensive quality evaluation was conducted by assessing root traits and quantifying the contents of nine compounds,using correlation analysis,principal component analysis,and the entropy weighted TOPSIS method.The results showed that QH003yyf(S2),TGT(S14),and ZH002QS(S18)were disease-free,and had large single root mass(0.97~1.54 kg),thick and long fibrous roots and reeds(31.72~36.00 mm,30.5~36.1 cm;58.32~68.47 mm,5.1~6.8 cm).The total contents of emodin,rhein,chrysophanol,aloeemodin,and physcion contained in roots of 18 R.tanguticum Maxim.were in accordance with Pharmacopoeia standards.QH002yyf(S16),DH002yyfA(S3),HS002yyf(S1),TGTYS(S6),TGT(S14),and ZH001DH(S8)were high in the total content of the nine compounds,in the order of 48.86,46.41,45.88,44.96,44.23,43.81 mg·g^(-1).The top 2 of the comprehensive ranking analyzed by entropy weight TOPSIS method were QH004yyf(S7)and ZH002QS(S18).In this study,TGT(S14)and ZH002QS(S18)were selected as the foundational materials for breeding and cultivating Rheum tanguticum varieties.The evaluation model,established through multi-indicator joint multivariate statistical analysis for screening different flower colors in Rheum tanguticum breeding materials,can also be applied to the screening and evaluation of other breeding materials.

关 键 词：唐古特大黄 育种材料筛选 花色 根部性状 多指标联合多元统计分析法 

分 类 号：S567.239[农业科学—中草药栽培]