NAT10作为肝癌潜在生物学标志物的生物信息学分析  

Bioinformatics Analysis of NAT10 As A Potential Biomarker for Hepatocellular Carcinoma

作　　者：王丹 姜可伊[1,2] 付松波 孙悦 WANG Dan;JIANG Keyi;FU Songbo(Institute of Keshan Disease,Center for Endemic Disease Control,Chinese Center for Disease Control and Prevention,Harbin Medical University,Heilongjiang 150081,China)

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心克山病防治研究所,150081 [2]国家卫生健康委病因流行病学重点实验室(哈尔滨医科大学)、黑龙江省普通高校病因流行病学重点实验室,150081

出　　处：《医学研究杂志》2025年第3期47-53,共7页Journal of Medical Research

基　　金：国家自然科学基金青年科学基金资助项目(82200304);黑龙江省省属高等学校基本科研业务费(2022-KYYWF-0260)。

摘　　要：目的探讨NAT10在肝癌中的临床意义与潜在作用机制。方法基于癌症基因组图谱(The Cancer Genome Atlas,TCGA)获得的50例正常和374例肿瘤样本,采用R 4.2.1处理所获数据。结果NAT10在肿瘤样本中的表达显著高于正常样本(P<0.001)。与NAT10低表达患者比较,NAT10高表达患者预后较差(P<0.001)。Logistic回归分析结果表明,NAT10高表达与肝癌患者临床预后不良因素相关,NAT10高表达患者更易于进展到晚期。基因集富集分析(gene set enrichment analysis,GSEA)结果显示,NAT10高表达样本中,异生物质代谢、凝血、脂肪酸代谢等相关基因特征均有差异富集。蛋白互作分析结果显示,NAT10可能与IGF2、SST、MUC2、CHGA、AGR2等基因存在相互作用。结论NAT10在肝癌中高表达,且表达水平与肝癌的临床特征及患者生存率存在相关性。NAT10可能是一种潜在的肝癌预后生物学标志物。Objective To investigate the clinical significance and potential mechanism of NAT10 in hepatocellular carcinoma.Methods A total of 50 normal and 374 tumor samples were obtained based on The Cancer Genome Atlas(TCGA),and the data were processed by R 4.2.1.Results The expression of NAT10 in tumor samples was significantly higher than that in normal samples(P<0.001).Compared with patients with low expression of NAT10,patients with high expression of NAT10had worse prognosis(P<0.001).Logistic regression analysis showed that high NAT10 expression was associated with adverse clinical prognostic factors,and patients with high NAT10 expression were more likely to progress to the advanced stage.Gene set enrichment analysis(GSEA)results showed that the genes related to heterobiotic metabolism,blood coagulation and fatty acid metabolism were differentially enriched in the samples with high expression of NAT10.The protein interaction analysis results showed that NAT10may interact with genes such as IGF2,SST,MUC2,CHGA,AGR2,etc.Conclusion NAT10 is highly expressed in hepatocellular carcinoma,and the expression level is correlated with the clinical characteristics and survival rate of patients with hepatocellular carcinoma.NAT10may be a potential molecular biomarker for hepatocellular carcinoma prognosis.

关 键 词：NAT10 肝癌 预后不良 生物学标志物 

分 类 号：R735[医药卫生—肿瘤]