CD13和FUT8在胃癌组织中的表达及与淋巴结转移的关系  

Expression of CD13 and FUT8 in gastric cancer and its correlation with lymph node metastasis

作　　者：汤小龙 向正国 陈旭峰 郑杨 杨胜兰 张骁 TANG Xiao-long;XIANG Zheng-guo;CHEN Xu-feng;ZHENG Yang;YANG Sheng-lan;ZHANG Xiao(Department of Gastroenterology,No.904 Hospital,Joint Logistics Support Force of Chinese People’s Liberation Army,Wuxi 214008,Jiangsu,China;Department of Internal Medicine-Cardiovascular,No.904 Hospital,Joint Logistics Support Force of Chinese People’s Liberation Army,Wuxi 214008,Jiangsu,China)

机构地区：[1]中国人民解放军联勤保障部队第九〇四医院消化内科,江苏无锡214008 [2]中国人民解放军联勤保障部队第九〇四医院心血管内科,江苏无锡214008

出　　处：《生物医学工程与临床》2025年第2期250-255,共6页Biomedical Engineering and Clinical Medicine

基　　金：无锡市科技发展基金项目(K20221041)。

摘　　要：目的探讨白细胞分化抗原13(CD13)、岩藻糖基转移酶8(FUT8)、CD13 mRNA、FUT8 mRNA在胃癌组织中的表达及其与淋巴结转移的相关性。方法选择2020年1月至2024年1月在中国人民解放军联勤保障部队第九〇四医院进行胃癌根治术的胃癌患者100例,其中男性62例,女性38例;年龄41~74岁,平均年龄59.03岁(标准差9.33岁);肿瘤直径≤2 cm 42例,>2 cm 58例;浸润深度,黏膜、黏膜下层12例,浆膜层26例,肌层+浆膜下层62例;组织学分型,分化型62例,未分化型38例;隆起或浅表型45例,溃疡或瘢痕型55例;胃部上1/332例,胃部中1/334例,胃部下1/334例;癌胚抗原(CEA)16.24~36.32μg/L,平均值23.14μg/L;糖类抗原199(CA199)82.54~98.55 kU/L,平均值90.54 kU/L。根据术后结果,分为淋巴结转移组(LN组)74例和非淋巴结转移组(非LN组)26例。对比两组患者临床资料,免疫组化、实时荧光定量聚合酶链式反应(qRT-PCR)及Western blot检测CD13、FUT8表达,采用Logistic回归分析影响淋巴结转移的危险因素。结果两组患者肿瘤浸润深度、组织学分型、是否合并溃疡、肿瘤直径比较,差异有统计学意义(P<0.05)。胃癌组织CD13、CD13 mRNA水平高于癌旁组织(CD13:LN组4.24±0.24 vs 1.00±0.04,非LN组2.04±0.15 vs 1.00±0.04;CD13 mRNA水平:LN组1.97±0.24 vs 0.34±0.03,非LN组0.99±0.08 vs 0.33±0.02),FUT8、FUT8 mRNA水平低于癌旁组织(FUT8:LN组0.17±0.04 vs 1.00±0.03,非LN组0.48±0.05 vs 1.00±0.03;FUT8 mRNA水平:LN组0.35±0.03 vs 1.21±0.02,非LN组0.76±0.06 vs 1.20±0.03)(P<0.05)。LN组癌组织CD13、CD13 mRNA水平高于非LN组癌组织(4.24±0.24 vs 2.04±0.15、1.97±0.24 vs 0.99±0.08),LN组癌组织FUT8、FUT8 mRNA水平低于非LN组癌组织(0.17±0.04 vs 0.48±0.05、0.35±0.03 vs 0.76±0.06)(P<0.05)。肿瘤浸润至浆膜层、组织学分型为未分化型、合并溃疡、CD13高表达及FUT8低表达是淋巴结转移的独立危险因素(P<0.05)。结论胃癌组织中CD13高表达和FUT8低表达均与胃�Objective To explore expression of cluster of differentiation 13(CD13),fucosyl transferase 8(FUT8),CD13 mRNA and FUT8 mRNA in gastric cancer tissues and their correlation with lymph node metastasis.Methods From January 2020 to January 2024,a total of 100 patients with gastric cancer performed radical gastrectomy for gastric cancer were enrolled,which included 62 males and 38 females,aged 41-74 years old with mean age of 59.03 years old(standard deviation 9.33 years old);cancer diameter was≤2 cm in 42 cases and>2 cm in 58 cases;12 cases of mucosa and submucosa,26 of serosa and 62 of muscular layer+subserosa;62 cases of histologically differentiated type and 38 of undifferentiated type;45 cases of protuberant or superficial phenotype,55 cases of ulcer or scar type;32 cases at the upper 1/3 of stomach,34 at the middle 1/3 of stomach and 34 at the lower 1/3 of the stomach.Carcinoembryonic antigen(CEA)was 16.24-36.32μg/L with mean of 23.14μg/L;carbohydrate antigen 199(CA199)was 82.54-98.55 kU/L with mean of 90.54 kU/L.According to postoperative results,all of them were divided into lymph node metastasis group(LN group,n=74)and non-lymph node metastasis group(non-LN group,n=26).The clinical data,immunohistochemistry,real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)and Western blot were used to detect the expression of CD13 and FUT8,and Logistic regression analysis was used to analyze the risk factors affecting lymph node metastasis.Results There were significant differences in the depth of cancer invasion,histological type,combined ulcer and tumor diameter between 2 groups(P<0.05).The levels of CD13 and CD13 mRNA in gastric cancer tissues were higher than those in adjacent tissues(CD13:LN group 4.24±0.24 vs 1.00±0.04,non-LN group 2.04±0.15 vs 1.00±0.04;CD13 mRNA level:LN group 1.97±0.24 vs 0.34±0.03,non-LN group 0.99±0.08 vs 0.33±0.02),FUT8 and FUT8 mRNA levels were lower than those in adjacent tissues(FUT8:LN group 0.17±0.04 vs 1.00±0.03,non-LN group 0.48±0.05 vs 1.00±0.03;FUT8 mRN

关 键 词：胃癌 淋巴结转移 CD13 FUT8 

分 类 号：R735.2[医药卫生—肿瘤]