孕酮和脂联素受体3多肽通过影响磷脂酰肌醇3-激酶/蛋白激酶B信号通路抑制胃癌细胞增殖和迁移  

Progesterone and adiponectin receptor 3 peptide inhibit gastric cancer cell proliferation and migration by affecting the phosphatidylinositol 3-kinase/protein kinase B signaling pathway

作　　者：李龙 吴盈利[3] 杜侠莹 王文静 李沛 凌志强[1,2] Li Long;Wu Yingli;Du Xiaying;Wang Wenjing;Li Pei;Ling Zhiqiang(Postgraduate Training Base Alliance of Wenzhou Medical University(Zhejiang Cancer Hospital),Hangzhou Institute of Medicine(HIM),Chinese Academy of Sciences,Hangzhou 310022,China;Department of Molecular Biology,Zhejiang Cancer Institute,Zhejiang Cancer Hospital,Hangzhou Institute of Medicine(HIM),Chinese Academy of Sciences,Hangzhou 310022,China;Department of Anesthesiology,Zhejiang Cancer Hospital,Hangzhou Institute of Medicine(HIM),Chinese Academy of Sciences,Hangzhou 310022,China;Zhejiang Second Clinical College of TCM University,Hangzhou 310053,China;Department of Pathophysiology,School of Basic Medicine,Zhengzhou University,Zhengzhou 450001,China)

机构地区：[1]温州医科大学研究生培养基地(浙江省肿瘤医院),中国科学院杭州医学研究所,杭州310022 [2]浙江省肿瘤医院浙江省肿瘤研究所分子生物学实验室,杭州310022 [3]浙江省肿瘤医院麻醉科,杭州310022 [4]浙江中医药大学第二临床学院,杭州310053 [5]郑州大学基础医学院病理生理学系,郑州450001

出　　处：《中华实验外科杂志》2025年第1期70-73,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金面上项目(32271238);国家卫生健康委科学研究基金-浙江省卫生健康重大科技计划重点项目(WKJ-ZJ-2117);浙江省公益技术研究计划实验动物项目(LGD20H160003)。

摘　　要：目的观察孕酮和脂联素受体3(PAQR3)多肽在体外抑制胃癌细胞增殖和迁移能力以及对磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)的影响。方法委托公司合成涵盖PAQR3第6-55个肽段的PAQR3多肽(P6-55);在人胃癌细胞系AGS细胞中加入PAQR3多肽和对照肽段,采用蛋白质印迹法(Western blot)、Real-time反转录-聚合酶链反应(RT-PCR)分别在蛋白和mRNA水平上观察PAQR3多肽(P6-55)对PI3K/Akt表达的影响,使用细胞计数试剂盒(CCK-8)细胞增殖实验和Transwell实验检查PAQR3多肽(P6-55)对胃癌细胞增殖和迁移的影响。结果CCK-8增殖实验结果显示,PAQR3多肽(P6-55)处理AGS细胞24、48、72 h后细胞数显著低于对照肽段处理组(0.388±0.021比0.456±0.037、0.635±0.033比0.726±0.048、0.893±0.051比1.226±0.071),差异均有统计学意义(t=5.236、5.967、8.767,P<0.05)。Transwell实验结果显示,PAQR3多肽(P6-55)处理AGS细胞24 h后细胞数显著少于对照肽段处理组[(125±15)个比(673±27)个],差异有统计学意义(t=16.653,P<0.05)。Real-time RT-PCR检测发现,PAQR3多肽(P6-55)处理组细胞中PI3K、Akt mRNA的表达水平显著低于对照肽段处理组(0.420±0.041比2.856±0.091、0.380±0.032比3.116±0.127),差异有统计学意义(t=19.358、21.218,P<0.05)。Western blot结果显示,PAQR3多肽(P6-55)处理组细胞中p-PI3K、p-Akt蛋白表达显著少于对照肽段处理组(0.290±0.019比0.891±0.032、0.190±0.014比0.790±0.023),差异有统计学意义(t=5.985、13.136,P<0.05)。结论PAQR3多肽(P6-55)可通过影响PI3K/Akt信号通路抑制胃癌细胞增殖和迁移。Objective To study the effects of progesterone and adiponectin receptor 3(PAQR3)peptide on gastric cancer cell proliferation and migration and on the expression of phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt).Methods PAQR3 peptide covering PAQR36th-55th peptide(P6-55)was synthesized.PAQR3 peptide and control peptide were added to human gastric cancer cell line AGS cells,and the effect of PAQR3 peptide(P6-55)on PI3K/Akt expression was observed at protein and mRNA levels by Western blotting and real-time reverse transcriptase-polymerase chain reaction(RT-PCR),respectively.The effect of PAQR3 peptide(P6-55)on cell proliferation and migration was observed using the cell counting kit(CCK-8)cell proliferation experiment and the Transwell experiment.Results The results of CCK-8 proliferation experiment showed that the number of AGS cells treated with PAQR3 peptide(P6-55)at 24,48 and 72 h was significantly less than that in the control peptide group(0.388±0.021 vs.0.456±0.037,0.635±0.033 vs.0.726±0.048,0.893±0.051 vs.1.226±0.071)(t=5.236,5.967,8.767,P<0.05).The results of the Transwell experiment showed that the number of AGS cells treated with PAQR3 peptide(P6-55)was significantly less than that treated with control peptide segment after 24 h[(125±15)cells vs.(673±27)cells,t=16.653,P<0.05].Real-time RT-PCR found that the expression levels of PI3K and Akt mRNA in cells treated with PAQR3 peptide(P6-55)were significantly lower than those in cells treated with control peptide(0.420±0.041 vs.2.856±0.091,0.380±0.032 vs.3.116±0.127)(t=19.358,21.218,P<0.05).The Western blotting results showed that the expression of p-PI3K and p-Akt protein in cells treated with PAQR3 peptide(P6-55)was significantly lower than that in cells treated with control peptide(0.290±0.019 vs.0.891±0.032,0.190±0.014 vs.0.790±0.023)(t=5.985,13.136,P<0.05).Conclusion PAQR3 peptide(P6-55)can inhibit the proliferation and migration of gastric cancer cells by affecting the PI3K/Akt signaling pathway.

关 键 词：孕酮和脂联素受体3多肽(P6-55) 胃癌 磷脂酰肌醇3-激酶/蛋白激酶B信号通路 增殖 迁移 

分 类 号：R735.2[医药卫生—肿瘤]