外周血piR-018569表达水平作为标志物预测胃癌肝转移的研究  

Serum piR-018569 expression level as a marker to predict gastric cancer liver metastasis

作　　者：吴伟星 杜义安 Wu Weixing;Du Yian(Postgraduate Training Base Alliance of Wenzhou Medical University(Zhejiang Cancer Hospital),Hangzhou 310022,China;Department of Gastroenterology,Zhejiang Cancer Hospital,Hangzhou 310022,China)

机构地区：[1]温州医科大学研究生培养基地(浙江省肿瘤医院),杭州310022 [2]浙江省肿瘤医院胃外科,杭州310022

出　　处：《中华实验外科杂志》2025年第1期104-107,共4页Chinese Journal of Experimental Surgery

基　　金：浙江省基础公益研究计划(LGF21H160010)。

摘　　要：目的检测胃癌患者外周血中piR-018569的表达,探讨其能否作为判断患者肝转移的标志物。方法实时反转录聚合酶联反应(real-time RT-PCR)检测2019年1月至2024年10月期间浙江省肿瘤医院100例胃癌患者及50例健康人外周血piR-018569表达,同时采用化学发光法测定胃癌患者血清癌胚抗原(CEA)及糖类抗原199(CA199)水平。组间比较采用配对样本t检验,计数资料以%(例)表示。结果胃癌患者组血清piR-018569表达水平显著高于健康人组(10.37±3.98比0.98±0.15),差异有统计学意义(t=0.879,P<0.05)。血清piR-018569表达水平与肿瘤分化(9.10±3.87比13.58±1.95)、Lauren分型(7.58±3.25比12.79±2.81)、浸润深度(7.99±3.56比12.69±2.85)、淋巴结转移(7.40±3.49比11.48±3.58)、肝转移(6.40±1.61比12.95±2.74)及临床分期(6.40±1.61比12.95±2.74)显著相关,差异有统计学意义(t=0.12、0.79、0.30、0.75、0.37、0.37,P<0.05);而与性别(10.11±4.00比10.35±3.72)、肿瘤诊断时年龄(10.41±3.88比10.31±4.18)及肿瘤部位(9.80±3.82比10.99±4.10)无明显相关,差异无统计学意义(t=0.60、0.58、0.33,P>0.05)。CEA、CA199阳性率与血清piR-018569表达均呈显著相关(6.51±1.83比13.58±1.83,5.85±0.90比13.07±2.28),差异有统计学意义(t=0.66、0.43,P<0.05)。结论piR-018569表达水平与胃癌进展密切相关,可作为诊断胃癌肝转移的潜在生物标志物。Objective The expression of piR-018569 in peripheral blood of gastric cancer patients was detected to explore whether it could be used as a marker to judge liver metastasis.Methods Real-time reverse transcription polymerase chain reaction(real-time RT-PCR)was used to detect the expression of piR-018569 in peripheral blood samples from 100 gastric cancer patients and 50 healthy individuals in Zhejiang Cancer Hospital between January 2019 and October 2024.The chemiluminescence was employed to measure serum carcinoembryonic antigen(CEA)and CA199 levels in gastric cancer patients.Paired sample t-test was used for inter-group comparison.The count data were expressed as%(cases).Results The serum piR-018569 expression levels in gastric cancer patients were significantly higher than those in healthy individuals(10.37±3.98 vs.0.98±0.15)(t=0.88,P<0.05).Serum piR-018569 expression levels were significantly associated with tumor differentiation(9.10±3.87 vs.13.58±1.95),Lauren classification(7.58±3.25 vs.12.79±2.81),depth of infiltration(7.99±3.56 vs.12.69±2.85),lymph node metastasis(7.40±3.49 vs.11.48±3.58),liver metastasis(6.40±1.61 vs.12.95±2.74),and clinical stage(6.40±1.61 vs.12.95±2.74)(t=0.12,0.79,0.30,0.75,0.37,0.37,P<0.05).There were no significant associations with gender(10.11±4.00 vs.10.35±3.72),age at diagnosis(10.41±3.88 vs.10.31±4.18),or tumor location(9.80±3.82 vs.10.99±4.10)(t=0.60,0.58,0.33,P>0.05).CEA and CA199 positivity rates were significantly correlated with serum piR-018569 expression(6.51±1.83 vs.13.58±1.83,5.85±0.90 vs.13.07±2.28)(t=0.66,0.43,P<0.05).Conclusion The expression level of serum piR-018569 in gastric cancer patients is closely related to the progression of gastric cancer and can be used as a potential biomarker for diagnosing gastric cancer metastasis.

关 键 词：胃癌 肝转移 癌胚抗原 糖类抗原199 

分 类 号：R735.2[医药卫生—肿瘤]