基于癌症基因组图谱数据库挖掘乳头状肾细胞癌预后相关基因  

Exploring prognosis-related genes of kidney renal papillary cell carcinoma based on The Cancer Genome Atlas databases

作　　者：马彦 马静 赵鹏程 田沛 冯超杰 Ma Yan;Ma Jing;Zhao Pengcheng;Tian Pei;Feng Chaojie(Department of Urology,Affiliated Cancer Hospital of Zhengzhou University,Henan Cancer Hospital,Zhengzhou 450008,China)

机构地区：[1]郑州大学附属肿瘤医院,河南省肿瘤医院泌尿外科,郑州450008

出　　处：《中华实验外科杂志》2025年第1期130-133,共4页Chinese Journal of Experimental Surgery

摘　　要：目的采用生物信息学方法对癌症基因组图谱(TCGA)数据库进行挖掘,寻找影响乳头状肾细胞癌(KIRP)患者预后的相关基因。方法TCGA中下载KIRP患者相关数据,应用RStudio软件筛选差异表达基因,cluster Profiler包对上调及下调表达前50的差异基因进行基因本体论(GO)及京都基因和基因组百科全书(KEGG)富集分析。用基因表达谱交互分析(GEPIA2)从差异基因中筛选出影响KIRP患者预后的基因,将筛选出的基因按表达量的中位数分为高低表达组,进行生存分析和多因素回归分析。应用χ2检验、Kaplan-Meier分析及Cox回归进行数据分析。结果筛选出差异表达基因2000个。差异基因GO及KEGG富集分析发现体液水平调节、金属离子跨膜转运体活性、神经活性配体受体相互作用等功能和通路有差异。生存分析结果显示,肿瘤分期、前列腺素E受体3(PTGER3)、泰克酪氨酸激酶(TEK)、菱形5同系物2(RHBDF2)、损伤特异性DNA结合蛋白2(DDB2)、减数分裂结构特异性核酸内切酶1(EME1)、E2F转录因子2(E2F2)、着丝粒蛋白A(CENPA)、母系胚胎亮氨酸拉链蛋白激酶(MELK)、运动神经元和胰腺同源框基因1(MNX1)、HtrA丝氨酸肽酶4(HTRA4)、磷酸化细胞分裂周期蛋白25C(CDC25C)、蛋白激酶膜相关酪氨酸/苏氨酸1(PKMYT1)、胆碱能受体烟碱ɑ1亚基(CHRNA1)、线粒体裂变调节因子2(MTFR2)、细胞分裂周期45(CDC45)、着丝粒蛋白H(CENPH)、ATP结合蛋白家族6(ABCB6)及富含亮氨酸重复蛋白20(LRRC20)影响KIRP患者OS(χ2=43.855、6.512、4.453、6.143、9.960、13.674、11.952、14.309、7.911、4.165、4.570、13.072、13.484、4.658、6.820、4.702、11.650、5.354、7.966,P<0.05)。将肿瘤分期及上述18个差异基因纳入多因素Cox回归分析,结果表明,肿瘤高分期[风险比(HR)=4.27,95%置信区间(CI)=1.83~10.00,P<0.01]、EME1(HR=5.58,95%CI=2.30~13.52,P<0.01)及CENPH高表达(HR=2.94,95%CI=1.22~7.07,P<0.05)是影响KIRP患者OS的独立危险因Objective To explore the prognosis-related genes of patients with kidney renal papillary cell carcinoma(KIRP)based on The Cancer Genome Atlas(TCGA)databases by bioinformatics method.Methods The data of patients with KIRP were downloaded from TCGA,and the differentially expressed genes(DEGs)were screened by RStudio software.The DEGs were enriched by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Gnomes(KEGG)by cluster Profile,and screened by Gene Expression Profiling Interactive Analysis 2(GEPIA2)for prognostic genes for KIRP.Genes were divided into high expression group and low expression group according to median expression levels,and survival analysis and multivariate regression analysis were performed.Chi-square test,Kaplan-Meier and Cox regression analysis were used for data analysis.Results A total of 2000 DEGs were screened.GO and KEGG analyses revealed regulation of body fluid,metal ion transmembrane transporter activity,neuroactive ligand-receptor interaction and other functions and pathways.Survival analysis showed that tumor stage,prostaglandin E receptor 3(PTGER3),TEK receptor tyrosine kinase(TEK),rhomboid 5 homolog 2(RHBDF2),damage specific DNA binding protein 2(DDB2),essential meiotic structure-specific endonuclease 1(EME1),E2F transcription factor 2(E2F2),Centromere protein A(CENPA),maternal embryonic leucine zipper kinase(MELK),motor neuron and pancreas homeobox 1(MNX1),HtrA serine peptidase 4(HTRA4),cell division cycle 25 homolog C(CDC25C),protein kinase membrane associated tyrosine/threonine 1(PKMYT1),Cholinergic receptor nicotinic alpha 1 subunit(CHRNA1),mitochondrial fission regulator 2(MTFR2),cell division cycle 45(CDC45),centromere protein H(CENPH),ATP binding cassette subfamily B member 6(ABCB6)and leucine rich repeat containing 20(LRRC20)affected overall survival(OS)in patients with KIRP(χ2=43.855,6.512,4.453,6.143,9.960,13.674,11.952,14.309,7.911,4.165,4.570,13.072,13.484,4.658,6.820,4.702,11.650,5.354,7.966,P<0.05).Multivariate Cox regression analysis showed that higher tumor stage

关 键 词：乳头状肾细胞癌 减数分裂结构特异性核酸内切酶1 着丝粒蛋白H DNA结合蛋白2 细胞分裂周期45 富含亮氨酸重复蛋白20 生物信息学 

分 类 号：R737.11[医药卫生—肿瘤]