The plant retromer components SNXs bind to ATG8 and CLASP to mediate autophagosome movement along microtubules  

作　　者：Yanglan Liao Xibao Li Wenlong Ma Xinyi Lin Jiayi Kuang Xuanang Zheng Zien Li Fanfan Qiao Chuanliang Liu Jun Zhou Faqiang Li Ruixi Li Byung-Ho Kang Hongbo Li Caiji Gao 

机构地区：[1]Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,School of Life Sciences,MOE Key Laboratory&Guangdong Provincial Key Laboratory of Laser Life Science,College of Biophotonics,South China Normal University,Guangzhou 510631,China [2]Centre for Cell&Developmental Biology and State Key Laboratory of Agrobiotechnology,School of Life Sciences,Chinese University of Hong Kong,Hong Kong,China [3]College of Life Sciences,South China Agricultural University,Guangzhou 510642,China [4]Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes,School of Life Sciences,Institute of Plant and Food Science,Southern University of Science and Technology,Shenzhen 518055,China

出　　处：《Molecular Plant》2025年第3期416-436,共21页分子植物(英文版)

基　　金：supported by grants from the National Natural Science Foundation of China(32270291,32061160467,32470797,and 31870171);the Guangdong Province Rural Revitalization Strategy Special Funding for Seed Industry Vitalization to C.G.,the NSFC grant(32000365,32370329);Guangdong Basic and Applied Basic Research Foundation(2021A1515010913,2024B1515020043);H.L.,and the Research Grant Council of Hong Kong(GRF14113921,GRF14109222,GRF14110823,N_CUHK462/22,and C4014-23G)to B.-H.K.

摘　　要：In eukaryotic cells,autophagosomes are double-membrane vesicles that are highly mobile and traffic along cytoskeletal tracks.While core autophagy-related proteins(ATGs)and other regulators involved in autophagosome biogenesis in plants have been extensively studied,the specific components regulating plant autophagosome motility remain elusive.In this study,using TurboID-based proximity labeling,we identify the retromer subcomplex comprising sorting nexin 1(SNX1),SNX2a,and SNX2b as interacting partners of ATG8.Remarkably,SNX proteins decorate ATG8-labeled autophagosomes and facilitate their coordinated movement along microtubules.Depletion of SNX proteins restricts the motility of autophagosomes in the cytoplasm,resulting in decreased autophagic flux.Furthermore,we show that the microtubule-associated protein CLASP is a bridge,connecting the SNX-ATG8-decorated autophagosomes to the microtubules.Genetically,the clasp-1 mutant phenotype resembles that of plants with disrupted SNXs or microtubule networks,displaying diminished autophagosome motility and reduced autophagic flux.Collectively,our study unveils a hitherto unanticipated role of the SNXs subcomplex in connecting autophagosomes with microtubules to promote autophagosome mobility in Arabidopsis.

关 键 词：autophagy SNX CLASP MICROTUBULE autophagosome trafficking Arabidopsis thaliana 

分 类 号：Q943[生物学—植物学] Q945.78