Probing PFOA-induced Metabolic Disorders in Tilapia Through Fish Skin Mucus  

作　　者：DONG Jiaxin SU Rui WU Xiaokang DONG Deming WANG Yining HUANG Chenni QIU Tao GU Yu HUA Xiuyi LIANG Dapeng 

机构地区：[1]Key Laboratory of Groundwater Resources and Environment of Ministry of Education,College of New Energy and Environment,Jilin University,Changchun 130012,P.R.China [2]State Key Laboratory of Inorganic Synthesis and Preparative Chemistry,College of Chemistry,Jilin University,Changchun 130012,P.R.China

出　　处：《Chemical Research in Chinese Universities》2025年第2期358-366,共9页高等学校化学研究(英文版)

基　　金：supported by the National Natural Science Foundation of China(No.22376084).

摘　　要：The study of the metabolic toxicity of perfluorooctanoic acid(PFOA)is of great importance in assessing its potential impact on aquatic organisms and the aquatic environment,and provides a fundamental scientific basis for environmental protection and sustainable development.In this study,an electrospray ionization(ESI)source capable of direct analysis of solution samples was combined with a high-resolution Orbitrap Fusion Tribrid mass spectrometer to investigate the metabolic perturbations induced by different concentrations of perfluorooctanoic acid(PFOA)and their correlations in skin mucus and liver tissue of tilapia.Seven common metabolites were found in mucus and liver,and nitrogen metabolism pathways were disturbed in all of them.The up-regulation of glutamate in mucus may reflect an increased demand for amino acids and energy,whereas the down-regulation of glutamate in the liver may lead to dysregulation of nitrogen metabolism,protein synthesis and amino acid metabolism.Enrichment analyses suggest a dysregulation of nitrogen metabolism pathways,which may lead to impaired maintenance of tissue function and dysregulated energy metabolism in tilapia.

关 键 词：Perfluorooctanoic acid(PFOA) Electrospray ionization-mass spectrometry(ESI-MS) Fish skin mucus Nitrogen metabolism 

分 类 号：X171.5[环境科学与工程—环境科学]