醛脱氢酶基因FvALDH-52和FvALDH-82对拟轮枝镰孢生长发育和致病力的影响  

The aldehyde dehydrogenase genes FvALDH-52 and FvALDH-82 affect the growth,development and pathogenicity of fungal pathogen Fusarium verticillioides

作　　者：贾慧[1] 任志国 张晓杰 刘宁[1] 孙蔓莉 曹志艳[1] 董金皋[1] Jia Hui;Ren Zhiguo;Zhang Xiaojie;Liu Ning;Sun Manli;Cao Zhiyan;Dong Jingao(Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology,State Key Laboratory of North China Crop Improvement and Regulation,College of Plant Protection,Hebei Agricultural University,Baoding 071000,Hebei Province,China)

机构地区：[1]河北农业大学植物保护学院,华北作物改良与调控国家重点实验室,河北省植物生理与分子病理学重点实验室,保定071000

出　　处：《植物保护学报》2025年第1期201-210,共10页Journal of Plant Protection

基　　金：国家现代农业产业技术体系专项(CARS-02);河北省重点研发计划(20326502D);河北省省级科技计划(23567601H)。

摘　　要：为明确醛脱氢酶(aldehyde dehydrogenase,ALDH)在玉米病原真菌拟轮枝镰孢Fusarium verticillioides生长发育及致病中的作用,利用实时荧光定量PCR(quantitative real-time polymerase chain reaction,qRT-PCR)技术分析拟轮枝镰孢的ALDH家族成员FvALDH-52和FvALDH-82基因在侵染玉米籽粒不同时期的表达水平,通过遗传转化方法对FvALDH-52和FvALDH-82进行基因敲除,分析2个基因在拟轮枝镰孢生长发育及致病性中的作用。结果表明:FvALDH-52和FvALDH-82在侵染玉米籽粒过程中表达水平均升高。敲除FvALDH-52和FvALDH-82基因后,拟轮枝镰孢的分生孢子产生能力降低,同时对H_(2)O_(2)和乙醛更加敏感,产生的伏马毒素显著降低;FvALDH-52基因敲除后导致病菌对玉米籽粒和玉米茎秆的致病力减弱。表明ALDH基因FvALDH-52在拟轮枝镰孢生长发育和致病过程中起着重要作用。To clarify the function of aldehyde dehydrogenase(ALDH)in the growth,development,and pathogenicity of the maize pathogenic fungus Fusarium verticillioides,quantitative real-time poly‐merase chain reaction(qRT-PCR)was employed to analyze the expression levels of ALDH family mem‐bers,the FvALDH-52 and FvALDH-82 genes of F.verticillioides,during different phases of its infec‐tion.Genetic transformation was carried out to knock out the FvALDH-52 and FvALDH-82 genes,with the aim of exploring the functions of these two genes in the growth,development,and pathogenicity of F.verticillioides.The results demonstrated that the expression levels of FvALDH-52 and FvALDH-82 in‐creased during the infection process of maize kernels.Following the knockout of FvALDH-52 and FvALDH-82,the sporulation capacity of F.verticillioides decreased,and it became more sensitive to H_(2)O_(2) and acetaldehyde,while the concentration of fumonitoxin declined substantially.The knockout of of FvALDH-52 weakened the pathogenicity of the pathogen towards kernels and stalks.These findings indicate that FvALDH-52 plays a crucial role in the growth, development, and pathogenicity of F. verti‐cillioides.

关 键 词：醛脱氢酶 拟轮枝镰孢 玉米病害 基因表达 功能分析 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]