负载姜黄素的玉米醇溶蛋白-海藻酸钠复合纳米颗粒的制备及表征  

Preparation and Characterization of Zein-Sodium Alginate Composite Nanoparticles Loaded with Curcumin

作　　者：刘心仪 于洋[1] 史晨杉 任媛媛[1] 毛继虎 张青旺 韩俊华[1] LIU Xinyi;YU Yang;SHI Chenshan;REN Yuanyuan;MAO Jihu;ZHANG Qingwang;HAN Junhua(College of Food Science and Biology,Hebei University of Science and Technology,Shijiazhuang 050018,China;Hebei Nainuo Technology Co.,Ltd.,Shijiazhuang 050000,China)

机构地区：[1]河北科技大学食品与生物学院,河北石家庄050018 [2]河北耐诺科技有限公司,河北石家庄050000

出　　处：《食品科技》2025年第2期236-243,共8页Food Science and Technology

基　　金：河北省重点研发计划项目(22321501D);河北省自然科学基金面上项目(C2022208017)。

摘　　要：为更好地发挥姜黄素的生物活性,研究制备递送姜黄素的纳米颗粒。文章以反溶剂沉淀法制备了负载姜黄素的玉米醇溶蛋白/海藻酸钠纳米颗粒(Zein-curcumin/Sodium alginate nanoparticles,Z-C/SA NPs),用于保护和递送姜黄素。以包埋率、粒径和多分散指数(Polydispersion index,PDI)为指标,确定了SA的最佳浓度,探究了纳米颗粒的光稳定性和抗氧化能力;通过改变pH值和盐浓度,探究了对其纳米颗粒粒径、PDI和Zeta电位的影响,并利用扫描电镜、红外光谱、X-射线、透射电镜对纳米颗粒进行表征。结果表明,当SA浓度为1.6 mg/mL时,制备的纳米颗粒包埋率最高为94.69%,粒径最小为239.36 nm,PDI指数均<0.4,ζ-电位为–33.43 mV,形态完整、表面光滑、结构致密。且Z-C/SA NPs包埋的姜黄素在紫外线照射2 h后的保留率达到69.12%,并且对p H3.0~7.0和Na Cl盐溶液具有良好的稳定性。综上所述,SA可以通过静电相互作用沉积在Zein纳米颗粒表面,为姜黄素提供更好的保护,形成更稳定的纳米颗粒。In order to better utilize the biological activity of curcumin,Zein/sodium alginate nanoparticles loaded with curcumin(Z-C/SA NPs)were prepared by anti-solvent precipitation method for the protection and delivery of curcumin.The optimal concentration of SA was determined according to embedding rate,particle size and polydispersion index(PDI).The photostability and antioxidant capacity of nanoparticles were explored.The effects of pH value and salt concentration on the particle size and Zeta potential of the nanoparticles were investigated,and the nanoparticles were characterized using scanning electron microscopy,infrared spectroscopy,X-ray,and transmission electron microscopy.The results showed that when the SA concentration was 1.6 mg/mL,the highest embedding rate of the prepared nanoparticles was 94.69%,and the minimum particle size was 239.36 nm,PDI index<0.4,theζ-potential was-33.43 mV.And the nanoparticles showed complete morphology,smooth surface,and dense structure.The retention rate of Z-C/SA NPs embedded curcumin reached 69.12%after 2 hours of ultraviolet radiation,and it had good stability to pH3.0~7.0 and NaCl salt solution.In conclusion,SA can be deposited on the surface of zein nanoparticles through electrostatic interaction to provide better protection for curcumin and form more stable nanoparticles.

关 键 词：纳米颗粒 姜黄素 玉米醇溶蛋白 海藻酸钠 

分 类 号：TS201.2[轻工技术与工程—食品科学]