miR-490-3p、miR-503-5p在肝细胞癌组织中的表达及与临床病理特征和预后的关系  

The expression of miR-490-3p and miR-503-5p in hepatocellular carcinoma tissues and their relationship with clinicopathological features and prognosis

作　　者：王军委 张小龙 李岩 刘峥[2] 张继光[1] 岳亚玲 WANG Jun-wei;ZHANG Xiao-long;LI Yan;LIU Zheng;ZHANG Ji-guang;YUE Ya-ling(Department of Interventional,Handan Central Hospital,Hebei 056000,China;Third Department of Oncology,Handan Central Hospital,Hebei 056000,China)

机构地区：[1]邯郸市中心医院介入科,河北056000 [2]邯郸市中心医院肿瘤三科,河北056000

出　　处：《肝脏》2025年第3期356-360,共5页Chinese Hepatology

基　　金：河北省2018年度医学科学研究重点课题计划(20181670)。

摘　　要：目的探讨肝细胞癌(HCC)中微小核糖核酸(miRNA)-490-3p、miR-503-5p表达及与临床病理特征和预后的关系。方法选取自2018年3月—2020年3月期间邯郸市中心医院接受根治性切除术的102例HCC患者。采用实时荧光定量聚合酶链式反应检测HCC癌组织和癌旁组织(距离癌组织边缘5 cm以上)中miR-490-3p、miR-503-5p表达。比较不同临床病理特征HCC患者癌组织中miR-490-3p、miR-503-5p表达差异。采用Kaplan-Meier法分析不同miR-490-3p、miR-503-5p表达HCC患者的总体生存情况,Log-Rank检验比较不同组别生存曲线差异。单因素和多因素COX风险回归模型分析HCC患者预后的影响因素。结果HCC癌组织中miR-490-3p、miR-503-5p的相对表达量分别为0.86±0.23、0.91±0.22,均低于癌旁组织(分别为1.90±0.31、2.12±0.41,均P<0.001)。HCC癌组织中miR-490-3p、miR-503-5p的表达与肿瘤分化程度、肿瘤最大径、中国肝癌分期(CNLC)有关(均P<0.05),与性别、年龄、肿瘤数目、HBsAg、术前甲胎蛋白无关,差异无统计学意义(均P>0.05)。miR-490-3p高表达组和低表达组3年总体生存率分别为84.62%(44/52)、48.00%(24/50),miR-503-5p高表达组和低表达组3年总体生存率分别为87.76%(43/49)、50.94%(27/53)。miR-490-3p低表达组、miR-503-5p低表达组3年累积生存率分别明显低于miR-490-3p高表达组、miR-503-5p高表达组,差异有统计学意义(Log-rankχ^(2)=16.231,10.604,P<0.001,0.001)。CNLC分期Ⅲa期、肿瘤最大径≥5 cm、低分化程度是影响HCC患者预后的独立危险因素,miR-490-3p、miR-503-5p表达升高是保护因素。结论HCC患者的miR-490-3p、miR-503-5p表达降低,两者与不良临床病理特征有关,是影响HCC患者预后的独立危险因素。Objective To investigate the expression of microRNA(miRNA)-490-3p and miR-503-5p in hepatocellular carcinoma(HCC) and their relationship with clinicopathological features and prognosis.Methods 102 HCC patients who underwent radical resection in Handan Central Hospital from March 2018 to March 2020 were selected.The expression of miR-490-3p and miR-503-5p in HCC tissues and adjacent tissues(more than 5 cm from the edge of cancer tissues) were detected by real-time fluorescence quantitative polymerase chain reaction.The expression of miR-490-3p and miR-503-5p in cancer tissues of HCC patients with different clinicopathological features was compared.The overall survival of HCC patients with different miR-490-3p and miR-503-5p expression were analyzed by Kaplan-Meier method.Log-Rank test was used to compare the differences in survival curves between different groups.Univariate and multivariate COX risk regression models were used to analyze the prognostic factors of HCC patients.Results The relative expression levels of miR-490-3p and miR-503-5p in HCC cancer tissues were(0.86±0.23) and(0.91±0.22) respectively,which were lower than those in cancer adjacent tissues(1.90±0.31) and(2.12±0.41),with a statistically significant difference(t=27.409,26.379,P=0.000,0.000).The expression of miR-490-3p and miR-503-5p in HCC tissues was related to the degree of tumor differentiation,the maximum diameter of tumor,and the Chinese liver cancer staging(CNLC),and the differences were statistically significant(all P<0.05).The expression of miR-490-3p and miR-503-5p was not related to gender,age,number of tumors,HBsAg and preoperative alpha-fetoprotein,and the difference was not statistically significant(all P>0.05).The 3-year overall survival rates of miR-490-3p high expression group and low expression group were 84.62%(44/52) and 48.00%(24/50) respectively.The 3-year overall survival rates of miR-503-5p high expression group and low expression group were 87.76%(43/49) and 50.94%(27/53) respectively.The 3-year cumulative survival

关 键 词：肝细胞癌 微小核糖核酸-490-3p 微小核糖核酸-503-5p 病理特征 预后 

分 类 号：R735.7[医药卫生—肿瘤]