微量营养素与肠易激综合征的孟德尔随机化研究  

Association between micronutrients and irritable bowel syndrome:a Mendelian randomization study

作　　者：章媛媛 徐鹤翔 李明[1,2] ZHANG Yuanyuan;XU Hexiang;LI Ming(Department of Anorectal Diseases,The First Affiliated Hospital of Anhui University of Chinese Medical,Hefei,Anhui 230601,China;Department of Anorectal Diseases,Shanghai University of Traditional Chinese Medicine Affiliated Shuguang Hospital Anhui Hospital,Hefei,Anhui 230031,China;Department of Infectious Diseases,The Third Affiliated Hospital of Anhui Medical University,Hefei,Anhui 230061,China)

机构地区：[1]安徽中医药大学第一附属医院肛肠科,安徽合肥230601 [2]上海中医药大学附属曙光医院安徽医院肛肠科,安徽合肥230031 [3]安徽医科大学第三附属医院感染科,安徽合肥230061

出　　处：《预防医学》2025年第4期378-381,共4页China Preventive Medicine Journal

基　　金：安徽省“十四五”省级中医优势专科建设项目(皖中医药服务秘-2021-71)。

摘　　要：目的采用孟德尔随机化(MR)方法探究微量营养素与肠易激综合征(IBS)的因果关系,为IBS防治提供依据。方法通过布里斯托大学MRC综合流行病学部门公开全基因组关联研究(GWAS)数据库和英国生物银行数据库收集14种微量营养素(铜、硒、锌、铁、镁、钙、钾、叶酸、维生素C、维生素D、维生素E、维生素B6、维生素B12和胡萝卜素)的GWAS资料,通过FinnGen R10数据库收集IBS的GWAS资料。采用两样本双向MR方法分析微量营养素与IBS的因果关系,以逆方差加权法为主要分析方法;采用Cochran Q检验评估工具变量间的异质性;采用MR-Egger回归法和MR-PRESSO检验评估水平多效性;采用留一法和漏斗图分析结果的稳定性。结果正向MR分析结果显示,维生素B12与IBS存在统计学关联(OR=1.523,95%CI:1.093~2.213),其他13种微量营养素与IBS未见统计学关联(均P>0.05);反向MR分析结果显示,IBS与14种微量营养素未见统计学关联(均P>0.05)。敏感性分析未发现工具变量间的异质性和水平多效性(均P>0.05),留一法和漏斗图提示结果稳健。结论高水平维生素B12与IBS风险增加有关,且未发现维生素B12与IBS的反向因果关系。Objective To explore the causal association between micronutrients and irritable bowel syndrome(IBS)using a Mendelian randomization(MR)method,so as to provide the evidence for formulating prevention and treatment measures for IBS.Methods Genome-wide association studies(GWAS)summary data for 14 micronutrients(copper,selenium,zinc,iron,magnesium,calcium,potassium,folate,vitamin C,vitamin D,vitamin E,vitamin B6,vitamin B12,and carotene)were collected from the MRC Integrative Epidemiology Unit at the University of Bristol GWAS data and the UK Biobank data.GWAS data for IBS were obtained from the FinnGen R10 database.A bidirectional two-sample MR analysis was conducted to assess the causal relationships between micronutrients and IBS,with the inverse-variance weighted method as the primary analytical approach.Heterogeneity among instrumental variables was evaluated using Cochran's Q test.Horizontal pleiotropy was assessed via MR-Egger regression and the MR-PRESSO test.The robustness of the results was examined using leave-one-out and funnel plot.Results Forward MR analysis revealed a statistically significant association between vitamin B12 and IBS(OR=1.523,95%CI:1.093-2.213),while no significant associations were observed for the other 13 micronutrients(all P>0.05).Reverse MR analysis showed no significant association between IBS and any of the 14 micronutrients(all P>0.05).Sensitivity analyses revealed no evidence of heterogeneity or horizontal pleiotropy among the instrumental variables(all P>0.05).The robustness of the findings was supported by leave-oneout and funnel plot.Conclusion Higher vitamin B12 level is associated with an increased risk of IBS,but no reverse causal relationship between vitamin B12 and IBS has been found.

关 键 词：肠易激综合征 微量营养素 孟德尔随机化 

分 类 号：R151.4[医药卫生—营养与食品卫生学]