一种改良的使用玻璃毛细管进行小鼠玻璃体腔注射方法  

An improved method for intravitreal injection in the experimental mouse eye with glass capillary

作　　者：卜诗淼 吴晓君 刘勇 石翔宇 张跃红 BU Shimiao;WU Xiaojun;LIU Yong;SHI Xiangyu;ZHANG Yuehong(Department of Ophthalmology,the Second Affiliated Hospital,South China University of Technology,Guangzhou 510006,China)

机构地区：[1]华南理工大学附属第二医院(广州市第一人民医院)眼科,广东广州510006

出　　处：《广州医药》2025年第3期350-355,共6页Guangzhou Medical Journal

基　　金：广东省自然科学基金面上项目(2023A1515010376);广州市科技计划项目(2023A03J0479)。

摘　　要：目的针对目前常规使用的玻璃体腔注射针头容易引起注射后小鼠眼内出血和损伤晶状体的缺陷,本研究采用直径仅0.08 mm的玻璃毛细管作为实验用小鼠眼内注射针头进行玻璃体腔注射,并评估其安全性和可行性。方法选取12只6-8周的C57BL/6J雄性小鼠,左眼注射磷酸盐缓冲液为实验组,右眼不做特殊处理为对照组。6只小鼠玻璃体腔注射后立即腹腔注射伊文思蓝,检测视网膜血管渗漏情况;另外6只小鼠玻璃体腔注射后24 h处死,视网膜铺片免疫荧光染色小胶质细胞特异性抗体抗离子钙接头蛋白1,分析小胶质细胞的形态变化。结果实验组和对照组血管与周围荧光强度比值分别为(4.45±0.30)和(4.51±0.24),小胶质细胞数量分别为(131.00±5.38)个/mm^(2)和(133.00±5.99)个/mm^(2),小胶质细胞胞体面积分别为34.02(27.82,40.54)μm^(2)和34.70(26.09,40.54)μm^(2),小胶质细胞分支长度分别为198.80(171.30,258.80)μm和223.30(178.20,278.30)μm,两组相比差异均无统计学意义(均P>0.05)。结论经改良的玻璃毛细管直径更细,损伤更小,可以替代传统的注射针头,可作为实验用小鼠眼内注射针头进行玻璃体腔注射。Objective To assess the safety and feasibility of employing an enhanced glass capillary,with a diameter of 0.08 mm,as an intraocular needle for intravitreal injections in experimental mice eyes.Methods Twelve male C57BL/6J mice,aged 6-8 weeks,were utilized in this investigation.Phosphate buffered saline(PBS)was administered via intravitreal injection into the left eye of each mouse(experimental group),while the right eye received no special treatment(control group).Six mice received an intraperitoneal injection of Evans blue immediately following intravitreal injection to detect retinal vessel leakage.The remaining six mice were euthanized 24 hours after intravitreal injection,and the retinas were subjected to immunofluorescence staining using a microglia-specific antibody to analyze morphological changes in microglia.Results In both the experimental and control groups,the ratio of vascular to peripheral fluorescence intensity was(4.45±0.30)and(4.51±0.24),respectively.The number of microglia was(131.00±5.38)/mm^(2) and(133.00±5.99)/mm^(2),the cell body area of microglia was 34.02(27.82,40.54)μm^(2) and 34.70(26.09,40.54)μm^(2),and the branch length of microglia was 198.80(171.30,258.80)μm and 223.30(178.20,278.30)μm,respectively.There were no statistically differences observed in any of the above indicators between the two groups(all P>0.05).Conclusions The use of this glass capillary,characterized by a narrower diameter,reduces tissue damage,demonstrates its potential to replace traditional injection needles for performing intravitreal injections in experimental mice.

关 键 词：玻璃体腔注射 玻璃毛细管 小胶质细胞 

分 类 号：R779.6[医药卫生—眼科]