宫颈高级别病变HPV感染亚型分布及血清miR-21、COX-2 mRNA、HLA-G和SCC-Ag表达水平  

Expressions of serum miR-21,COX-2 mRNA,HLA-G and SCC-Ag in high-grade cervical lesions patients with HPV subtypes infections

作　　者：刘维 曾宇晖 曾有星 LIU Wei;ZENG Yuhui;ZENG Youxing(Ganzhou Maternal and Child Health Hospital,Ganzhou,Jiangxi 341000,China)

机构地区：[1]赣州市妇幼保健院妇科,江西赣州341000

出　　处：《中华医院感染学杂志》2025年第5期697-702,共6页Chinese Journal of Nosocomiology

基　　金：江西省重点研发计划基金资助项目(20203BBGL75033)。

摘　　要：目的探讨宫颈高级别病变人乳头瘤病毒(HPV)感染亚型分布及其血清微小核糖核酸(miR)-21、环氧合酶-2(COX-2)mRNA、人白细胞抗原-G(HLA-G)和鳞状上皮细胞癌抗原(SCC-Ag)表达。方法采用随机数字表法选取2022年1月-2024年1月赣州市妇幼保健院收治的51例宫颈高级别病变HPV感染患者为感染组,52例宫颈高级别病变未感染HPV患者为未感染组,另选同期54名健康体检者为健康组。分析宫颈高级别病变HPV感染亚型及感染类型分布情况,比较各组血清miR-21、COX-2 mRNA、HLA-G和SCC-Ag水平,分析其对宫颈高级别病变HPV感染的诊断价值。结果感染组检测发现HPV感染例次为75例次,单一感染占比较多的为HPV16、HPV18,多重感染占比较多的为HPV16、HPV52和HPV58。感染组、未感染组和健康组血清miR-21、COX-2 mRNA、HLA-G和SCC-Ag水平比较,差异有统计学意义(P<0.05),四指标感染组高于未感染组和健康组(P<0.05),且未感染组高于健康组(P<0.05)。四指标联合检测对宫颈高级别病变HPV感染患者的诊断曲线下面积(AUC)值高于单独检测的AUC值(P<0.05),且联合检测的敏感度为92.16%,特异度为92.32%。结论HPV基因型HPV16在宫颈高级别病变HPV感染患者中最常见,且在单一及多重感染中均占比较高。miR-21、COX-2 mRNA、HLA-G和SCC-A与宫颈高级别病变有关,且与宫颈高级别病变HPV感染有关,四指标均呈高表达,且四指标联合检测对宫颈高级别病变HPV感染诊断价值较高。OBJECTIVE To explore the subtypes of human papillomavirus(HPV)infection and expressions of serum microribonucleic acid(miR)-21,cyclooxygenase-2(COX-2)mRNA,human leukocyte antigen-G(HLA-G)and squamous cell carcinoma antigen(SCC-Ag)in the patients with high-grade cervical lesions.METHODS Totally 51 high-grade cervical lesions patients with HPV infection who were treated in Ganzhou Maternal and Child Health Hospital from Jan.2022 to Jan.2024 were randomly selected as the infection group,52 high-grade cervical lesions patients who did not have HPV infection were chosen as the no infection group,meanwhile,54 healthy people who received physical examination were assigned as the healthy group.The subtypes of HPV infections and the types of infections were observed.The levels of serum miR-21,COX-2 mRNA,HLA-G and SCC-Ag were compared among the groups.The values of the indexes in diagnosis of HPV infection in the patients with high-grade cervical lesions were analyzed.RESULTS Totally 75 case-times of HPV infections were detected in the infection group;the patients with HPV16 infection or HPV18 infection were dominant among the patients with single subtype of HPV infection.The patients with mixed infections of HPV16,HPV52 and HPV58 were dominant among the patients with multiple subtypes of HPV infection.There were significant differences in the levels of serum miR-21,COX-2 mRNA,HLA-G and SCC-Ag among the infection group,the no infection group and the healthy group(P<0.05);the levels of the four indexes of the infection group were higher than those of the no infection group and the healthy group(P<0.05);the levels of the four indexes of the no infection group were higher than those of the healthy group(P<0.05).The area under the curve(AUC)value of the joint detection of the four indexes was higher than that of the single detection in diagnosis of the HPV infection in the patients with high-grade cervical lesions(P<0.05),the sensitivity of the joint detection was 92.16%,with the specificity 92.32%.CONCLUSIONS The HPV genotype

关 键 词：宫颈高级别病变 人乳头瘤病毒 感染 微小核糖核酸-21 环氧合酶-2 人白细胞抗原-G 鳞状上皮细胞癌抗原 

分 类 号：R737.33[医药卫生—肿瘤]