肺癌患者外周血T淋巴细胞PD-1及CD28表达特征分析  

Detection and clinical significance of PD-1/CD28 on the surface of peripheral blood T lymphocytes in patients with lung cancer

作　　者：黑若萱 李思敏 刘丽 董轲 陈延伟 郜赵伟 HEI Ruoxuan;LI Simin;LIU Li;DONG Ke;CHEN Yanwei;GAO Zhaowei(Department of Clinical Laboratory,Tangdu Hospital of the Air Force Medical University,Shaanxi Xi'an 710038,China;Department of Respiratory Medicine,Tangdu Hospital of the Air Force Medical University,Shaanxi Xi'an 710038,China)

机构地区：[1]空军军医大学唐都医院检验科,陕西西安710038 [2]空军军医大学唐都医院呼吸内科,陕西西安710038

出　　处：《现代肿瘤医学》2025年第5期772-776,共5页Journal of Modern Oncology

基　　金：国家自然科学基金面上项目(编号:82070096)。

摘　　要：目的:探讨肺癌患者外周血T淋巴细胞表面PD-1及CD28表达水平变化。方法:纳入2024年1月至2024年12月就诊于我院662例肺癌患者,其中包括556例肺癌未转移患者,66例肺癌伴转移患者。将同期送检的39例健康人作为对照组。采用流式细胞术检测外周血PD-1/CD28阳性的T淋巴细胞数量及比率。结果:与健康对照组相比,肺癌患者T淋巴细胞(CD45^(+)CD3^(+))和辅助性T淋巴细胞(CD45^(+)CD3^(+)CD4^(+))数量显著降低(P<0.05)。并且,与肺癌未转移组相比,肺癌伴转移组患者T淋巴细胞和辅助性T淋巴细胞数量显著降低(P<0.05)。与健康对照组相比,CD3^(+)PD-1^(+)T淋巴细胞比率在肺癌患者中显著升高(P<0.05),而在肺癌伴转移与未转移患者中无显著差异(P=0.303)。肺癌未转移组样本中CD3^(+)CD4^(+)PD-1^(+)T淋巴细胞比率和数量均显著高于健康对照组(P<0.05),肺癌转移组CD3^(+)CD4^(+)PD-1^(+)T细胞比率显著升高(P<0.05)。与健康对照组和肺癌未转移组相比,肺癌转移组中CD3^(+)CD8^(+)PD-1^(+)T淋巴细胞比率显著升高(P<0.05)。与健康对照组相比,肺癌患者CD3^(+)CD28^(+)、CD3^(+)CD4^(+)CD28^(+)以及CD3^(+)CD8^(+)CD28^(+)T淋巴细胞数量均显著降低(P<0.05)。结论:肺癌患者外周血中PD-1^(+)T淋巴细胞比率上调,而CD28^(+)T淋巴细胞数量下降,表明肺癌患者T细胞免疫功能受抑,可能与肺癌的发生发展密切相关。Objective:To investigate the alterations in the expression levels of PD-1 and CD28 on the surface of T lymphocytes present in the peripheral blood of lung cancer patients.Methods:A total of 662 lung cancer patients who visited our hospital from January 2024 to December 2024 were included in this study,comprising 556 patients with non-metastatic lung cancer and 66 patients with metastatic lung cancer.Additionally,a control group was established consisting of 39 healthy individuals examined during the same period.Flow cytometry was employed to assess both the quantity and proportion of PD-1/CD28 positive T lymphocytes within the peripheral blood samples.Results:Compared to the healthy control group,lung cancer patients exhibited a significant decrease in both the number of T lymphocytes(CD45^(+)CD3^(+))and helper T lymphocytes(CD45^(+)CD3^(+)CD4^(+))(P<0.05).Furthermore,when comparing the lung cancer metastasis group to the non-metastasis group,there was also a notable reduction in the number of T lymphocytes and helper T lymphocytes(P<0.05).In addition,relative to the healthy control group,lung cancer patients demonstrated a significant increase in the ratio of CD3^(+)PD-1^(+)T lymphocytes(P<0.05);however,no significant difference was observed between the metastasis and non-metastasis groups regarding this ratio(P=0.303).The ratio and quantity of CD3^(+)CD4^(+)PD-1^(+)T lymphocytes were significantly higher in the lung cancer non-metastasis group compared to those in the healthy control group(P<0.05),while an increased ratio of CD3^(+)CD4^(+)PD-1^(+)T cells was noted within the lung cancer metastasis group as well(P<0.05).Moreover,when contrasting with both healthy controls and individuals from the lung cancer non-metastasis cohort,there was a marked increase in the ratio of CD3^(+)CD8^(+)PD-1^(+)T lymphocytes within the lung cancer metastasis group(P<0.05).Lastly,compared to healthy controls,there was a significant reduction in numbers of CD3^(+)CD28^(+),CD3^(+)CD4^(+)CD28^(+),and CD3^(+)CD8^(+)CD28^(+)T lymphocy

关 键 词：肺癌 T淋巴细胞 PD-1 CD28 

分 类 号：R734.2[医药卫生—肿瘤]