PRKD3在结直肠癌组织中的表达及临床意义  

Expression of PRKD3 in colorectal cancer tissue and its clinical significance

作　　者：张斌[1] 常晨 陈敬礼 曹悦 常玉 刘乐乐 李文生[2] 王国荣[3] ZHANG Bin;CHANG Chen;CHEN Jingli;CAO Yue;CHANG Yu;LIU Lele;LI Wensheng;WANG Guorong(Xi'an Medical University,Shaanxi Xi'an 710068,China;Department of Pathology,Shaanxi Provincial People's Hospital,Shaanxi Xi'an 710068,China;The First Department of General Surgery,Shaanxi Provincial People's Hospital,Shaanxi Xi'an 710068,China)

机构地区：[1]西安医学院,陕西西安710068 [2]陕西省人民医院病理科,陕西西安710068 [3]陕西省人民医院普外一科,陕西西安710068

出　　处：《现代肿瘤医学》2025年第5期800-806,共7页Journal of Modern Oncology

基　　金：陕西省科技厅创新能力支撑计划-科技创新团队(编号:2020TD-048);陕西省人民医院科技人才支持计划(编号:2021LJ-08);陕西省人民医院科技发展孵化基金项目(编号:2021YJY-41)。

摘　　要：目的:检测蛋白激酶D3(protein kinase D3,PRKD3)在结直肠癌组织中的表达情况并探讨其临床意义。方法:使用生物信息学对PRKD3的表达情况及其与临床病理学指标之间进行相关性分析。并采用组织芯片-免疫组织化学染色法,检测81例结直肠癌组织及其配对的癌旁正常组织中PRKD3的蛋白表达情况,对PRKD3的蛋白表达情况与患者临床病理学指标之间的情况及患者预后生存情况进行相关性分析。采用qPCR法检测8例结直肠癌组织及其配对的癌旁正常组织中PRKD3的mRNA表达情况。结果:生物信息学分析发现相比较正常的结直肠组织,PRKD3 mRNA在结直肠癌组织中的表达水平明显下降(P<0.05),并且其表达与淋巴管侵犯、结肠息肉病史显著相关(P<0.05)。免疫组织化学染色结果显示PRKD3蛋白主要定位于结直肠细胞中的细胞质。其在癌旁正常组织中的阳性表达率为26.4%(19/72),而在结直肠癌组织中的阳性表达率为63.0%(51/81),两者之间有统计学差异(P<0.05)。qPCR结果显示与结直肠癌组织相比,癌旁正常组织中的PRKD3 mRNA表达显著升高。PRKD3的蛋白表达水平与临床诊断(结、直肠癌)具有相关性(P<0.05)。Kaplan-Meier生存曲线显示,PRKD3高表达患者的术后生存时间显著高于低表达患者(P<0.05)。多因素COX回归分析显示,PRKD3的高表达不是结直肠癌患者预后的独立因素。结论:PRKD3可能参与结直肠癌的发生发展,其可以作为结直肠癌患者预后及治疗的潜在分子标志。Objective:To investigate the expression of protein kinase D3(PRKD3)in colorectal cancer tissue and its clinical significance.Methods:Bioinformatics was used to analyze the expression of PRKD3 and its correlation with clinicopathological parameters.The protein expression of PRKD3 in 81 cases of colorectal cancer tissue and paired adjacent normal tissue was detected by tissue microarray and immunohistochemical staining.The correlation between the protein expression of PRKD3 and the clinicopathological parameters as well as the prognosis of the patients was analyzed.qPCR was used to detect the expression of PRKD3 mRNA in 8 cases of colorectal cancer tissue and paired adjacent normal tissue.Results:Bioinformatics analysis showed that the expression level of PRKD3 mRNA in colorectal cancer tissue was significantly lower than that in normal colorectal tissue(P<0.05),and its expression was significantly correlated with lymphatic vessel invasion and the history of colon polyps(P<0.05).Immunohistochemical staining showed that PRKD3 protein was mainly located in the cytoplasm of colorectal cells.The positive expression rate of PRKD3 in adjacent normal tissue was 26.4%(19/72),and that in colorectal cancer tissue was 63.0%(51/81).There was a statistical difference between the two groups(P<0.05).qPCR results showed that PRKD3 mRNA expression in adjacent normal tissue was significantly higher than that in colorectal cancer tissue.The protein expression level of PRKD3 was correlated with the clinical diagnosis of colorectal cancer(P<0.05).Kaplan-Meier survival curve showed that the postoperative survival time of patients with high PRKD3 expression was significantly longer than that of patients with low PRKD3 expression(P<0.05).Multivariate COX regression analysis showed that the high expression of PRKD3 was not an independent factor for the prognosis of colorectal cancer patients.Conclusion:PRKD3 may be involved in the occurrence and development of colorectal cancer,and it can be used as a potential molecular marker for the pro

关 键 词：结直肠癌 PRKD3 诊断 预后 

分 类 号：R735.3[医药卫生—肿瘤]