蔬菜中啶虫脒残留免疫分析方法研究  

Study on Immunoassay for Acetamiprid Residue in Vegetables

作　　者：刘蓓蓓 吕军[2] 张杰[1] 刘广洋 翟荣启 陈鸽 徐东辉[2] LIU Beibei;LYU Jun;ZHANG Jie;LIU Guangyang;ZHAI Rongqi;CHEN Ge;XU Donghui(College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,State Key Laboratory of Vegetable Biobreeding,Ministry of Agriculture and Rural Aff airs of Vegetable Product Quality Safety Risk Assessment Laboratory,Beijing 100081,China)

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,农业农村部蔬菜质量安全控制重点实验室,北京100081

出　　处：《中国蔬菜》2025年第4期126-131,共6页China Vegetables

基　　金：国家自然科学基金项目(32302207);国家重点研发计划项目(2022YFF0606800);国家现代农业技术产业体系建设专项基金项目(CARS-23-E03)。

摘　　要：为短期内快速监测蔬菜中啶虫脒的残留状况,建立了基于抗原抗体特异性结合的快速、简便的免疫分析方法。结果表明,该方法的检出限(LOD)为1.41μg·L^(-1),线性范围为2.35~50.12μg·L^(-1),灵敏度为10.86μg·L^(-1),在大白菜、西葫芦和黄瓜中的平均回收率为76.02%~116.71%,相对标准偏差(RSD)为0.92%~13.15%。此外,对比该免疫分析方法和液相色谱串联质谱法(LC-MS/MS),其检测结果的符合性较高。上述结果证明,该方法灵敏度高、准确性好、精密度高,能够实现蔬菜中啶虫脒的痕量检测。To rapidly monitor the residue status of acetamiprid in vegetables in short term,a rapid and simple immunoassay based on the specific combination of antigen and antibody was established.The results demonstrated that the assay has a limit of detection(LOD)of 1.41μg·L^(-1),a linear range of 2.35-50.12μg·L^(-1),and a sensitivity of 10.86μg·L^(-1).The average recovery rates in Chinese cabbage,zucchini,and cucumber were 76.02%-116.71%with relative standard deviation(RSD)ranging from 0.92%to 13.15%.Furthermore,comparing the immunoassay with the liquid chromatography-tandem mass spectrometry(LCMS/MS),the conformity of the results was high.The results demonstrate that the proposed immunoassay showed high sensitivity,accuracy and precision,enabling the trace detection of acetamiprid in vegetable s.

关 键 词：啶虫脒 蔬菜 免疫分析方法 农药残留 LC-MS/MS 

分 类 号：S482.2[农业科学—农药学] S482.3[农业科学—植物保护]