五行音乐对孕期恐应激子代抑郁行为及肠道菌群的影响  

Effects of five-element music on depressive behaviors and intestinal flora in offspring of stress-injured pregnant rats

作　　者：李笑琳 杨丽萍[1] 侯俊林[1] 王耀辉 余晨阳 李玲玲[2] LI Xiaolin;YANG Liping;HOU Junlin;WANG Yaohui;YU Chenyang;LI Lingling(Traditional Chinese Medicine School,Henan University of Chinese Medicine,Zhengzhou 450046,China;the First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000)

机构地区：[1]河南中医药大学中医学院,郑州450046 [2]河南中医药大学第一附属医院,郑州450000

出　　处：《中国比较医学杂志》2025年第2期13-23,共11页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(81973596);河南省中医药科学研究专项课题(2023ZY2032,2024ZY1001);河南省卫生健康委国家中医药传承创新中心科研专项(2023ZXZX1013)。

摘　　要：目的探究五行音乐对孕期恐应激子代抑郁样行为和肠道菌群的影响。方法将36只孕0.5 d Wistar雌鼠随机分为空白组、恐应激组和五行音乐组,所产仔鼠延续母代分组。孕1~19 d恐应激组和五行音乐组孕鼠采用旁观电击法造模,同时五行音乐组予五行音乐羽调干预。采用旷场实验、酶联免疫吸附法(ELISA)检测孕20 d孕鼠恐惧行为及血清糖皮质激素(glucocorticoid,GC)含量评价造模效果;采用旷场、悬尾和糖水偏好实验评价3周龄仔鼠抑郁情绪;采用高效液相检测仔鼠海马神经递质去甲肾上腺素(norepinephrine,NE)、多巴胺(dopamine,DA)和5-羟色胺(5-hydroxytryptamine,5-HT)水平;采用16S rRNA测序分析仔鼠肠道菌群变化规律;采用蛋白质免疫印迹(Western blot)实验检测仔鼠近端结肠蛋白Claudin1、Occludin和ZO1的表达。结果与空白组相比,恐应激组孕鼠旷场周围区域停留时间延长(P<0.05),进入中心区域次数减少(P<0.001),血清GC水平升高(P<0.001),而五行音乐组逆转了恐应激组的行为学变化(P<0.05)及血清GC水平(P<0.001)。相较空白组,恐应激组仔鼠旷场穿格次数减少(P<0.01)、糖水偏好指数降低、悬尾不动时间延长(P<0.05),海马内NE、DA、5-HT显著减少(P<0.05),肠道菌群多样性下降(P<0.01),菌群结构显著改变,其中变形菌门、肠杆菌科、肠球菌属、埃希氏菌属等丰度上调(P<0.05),螺旋体门、螺旋体科、毛螺菌科、瘤胃球菌科、密螺旋体属、普雷沃氏菌属、粪球菌属、异杆菌属、瘤胃球菌属、多尔氏菌属等丰度下调(P<0.05),近端结肠蛋白Claudin1、Occludin和ZO1表达量显著下调(P<0.05);相较恐应激组,五行音乐组仔鼠穿格次数、糖水偏好指数回升、悬尾不动时间缩短(P<0.05),海马内DA、5-HT显著回升(P<0.05),肠道菌群物种多样性增加(P<0.01),且逆转了上述菌群丰度变化,近端结肠蛋白Occludin和ZO1表达量显著上调(P<0.05)。结论孕期五行音乐�Objective To investigate the effects of five-element music on depressive behaviors and intestinal flora in offspring of fear-stress rats during pregnancy.MethodsThirty-six 0.5-day pregnant Wistar rats were divided randomly into a control group,fear-stress group,and five-element music group,and the subsequent litters continued the maternal grouping.Pregnant rats in the fear-stress and five-element music groups were modeled on days 1~19 of gestation using the bystander electric method,while the five-element music group was also exposed to Feather Tune five-element music intervention.The fear behavior and serum glucocorticoid(GC)levels in pregnant rats were assessed on day 20 of pregnancy by open field test and enzyme-linked immunosorbent assay,respectively,to evaluate the effectiveness of the model.Depression in 3-week-old offspring was evaluated by open field,tail suspension,and sucrose preference tests.Norepinephrine(NE),dopamine(DA),and 5-hydroxytryptamine(5-HT)levels in the hippocampus of the offspring were measured by high-performance liquid chromatography.Changes in the intestinal flora of the offspring were analyzed by 16S rRNA sequencing.Expression levels of the proximal colonic proteins claudin1,occludin,and ZO1 in the offspring were measured by Western blot assay.ResultsPregnant rats in the fear-stress group stayed longer in the area around the open field(P<0.05),had fewer entries into the central area(P<0.001),and had higher serum GC levels compared with those in the blank group(P<0.001).In contrast,five-element music exposure reversed these behavioral changes(P<0.05)and serum GC levels(P<0.001)in the fear-stress group.Offspring in the fear-stress group exhibited decreased open-field crossing frequency(P<0.01),reduced sucrose preference index,and longer immobility time in the tail suspension test(P<0.05),compared with those in the blank group,together with significantly decreased NE,DA,and 5-HT levels in the hippocampus(P<0.05).Rats in the fear-stress group showed decreased diversity of the intestinal fl

关 键 词：五行音乐 恐应激 子代 抑郁 肠道菌群 

分 类 号：R-33[医药卫生]