质子泵抑制剂泮托拉唑促进幽门螺旋杆菌SS1在小鼠胃内的定植  

Proton pump inhibitor pantoprazole promotes colonization of Helicobacter pylori Sydney strain 1 in the mouse stomach

作　　者：闫硕 李雪 王超[1] 徐嘉莉 程俣 张丽萍 苏磊 龚佳男 YAN Shuo;LI Xue;WANG Chao;XU Jiali;CHENG Yu;ZHANG Liping;SU Lei;GONG Jianan(National Center of Technology Innovation for Animal Model,National Human Diseases Animal Model Resource Center,NHC Key Laboratory of Human Disease Comparative Medicine,the Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100021,China)

机构地区：[1]中国医学科学院医学实验动物研究所,中国医学科学院&北京协和医学院人类疾病比较医学国家重点实验室,国家人类疾病动物模型资源中心,国家动物模型技术创新中心,北京100021

出　　处：《中国比较医学杂志》2025年第2期24-32,共9页Chinese Journal of Comparative Medicine

基　　金：中国医学科学院中央级公益性科研院所基本科研业务费专项(2021-RC310-008,2023-PT180-01);北京市科技新星项目(Z211100002121034);国家自然科学基金青年项目(82302985)。

摘　　要：目的寻找提高幽门螺旋杆菌(Helicobacter pylori,Hp)在小鼠胃部定植效率的方法。明确质子泵抑制剂(proton pump inhibitor,PPI)泮托拉唑是否能够作为定植辅助剂促进Hp在小鼠胃部定植,为建立Hp感染小鼠模型奠定基础。方法建立Hp悉尼株(Sydney strain 1,SS1)固体平板和液体培养体系并进行鉴定;比较不同剂量泮托拉唑对小鼠胃酸分泌的抑制效果;利用快速脲酶检测实验、细菌平板培养和TaqMan qPCR分析单独接种Hp或联合泮托拉唑预处理对Hp定植效率的影响。结果PPI预处理能够抑制胃酸分泌在一定程度上促进Hp在小鼠胃部定植。结论PPI能够作为定植辅助剂提高Hp感染小鼠模型的构建效率。Objective To explore method for improving the colonization efficiency of Helicobacter pylori(Hp)in the mouse stomach and to determine if the proton pump inhibitor(PPI)pantoprazole can act as a colonization adjuvant to enhance Hp colonization,with the aim of providing an effective tool for establishing an Hp infection mouse model.MethodsThe Hp Sydney strain 1(SS1)was introduced and solid plate and liquid culture systems were established.The effects of different doses of pantoprazole on gastric acid secretion in mice were compared.The impact of Hp inoculation,alone or combined with pantoprazole pretreatment,on Hp colonization efficiency was analyzed using rapid urease tests,bacterial plate cultures,and TaqMan quantitative polymerase chain reaction.ResultsPPI pretreatment inhibited gastric acid secretion and promoted Hp colonization in the mouse stomach,to some extent.Conclusions PPI can serve as colonization adjuvants to enhance the efficiency of constructing Hp infection mouse models.

关 键 词：幽门螺旋杆菌 质子泵抑制剂 幽门螺旋杆菌感染 小鼠模型 

分 类 号：R-33[医药卫生]