昼夜节律对慢性束缚应激小鼠焦虑、抑郁及认知行为的影响  

Effects of circadian rhythm on anxiety,depression,and cognitive behavior in mice with chronic restraint stress

作　　者：强萌 姜宁 黄红 张亦文 陈芳 李召辉 刘新民[3] 吕光华[1] QIANG Meng;JIANG Ning;HUANG Hong;ZHANG Yiwen;CHEN Fang;LI Zhaohui;LIU Xinmin;LYU Guanghua(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Institute of Medicinal Plant Development(IMPLAD),Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193;Institute of Drug Discovery Technology,Ningbo University,Ningbo 315000)

机构地区：[1]成都中医药大学药学院,成都611137 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193 [3]宁波大学新药技术研究院,浙江宁波315000

出　　处：《中国比较医学杂志》2025年第2期45-57,共13页Chinese Journal of Comparative Medicine

基　　金：四川省科技计划项目(2021YFYZ0012);中国医学科学院创新工程重大创新项目(2021-I2M-1-034);国家自然科学基金(82274056);新疆维吾尔自治区重大科技专项项目(2023A02010-3)。

摘　　要：目的探讨白天和夜晚两个不同束缚时段的慢性束缚应激28 d对雌雄ICR小鼠情绪及认知样行为的影响,为慢性束缚应激模型中实验动物性别和束缚时段的选择提供依据。方法将72只雌雄各半的ICR小鼠分为6组:雄性空白组、雄性白天束缚组、雄性夜间束缚组、雌性空白组、雌性白天束缚组、雌性夜间束缚组。除空白组外,其余各组每天束缚10 h,连续束缚28 d建立慢性束缚应激模型。通过旷场实验、Y迷宫实验、新奇抑制摄食实验、高架十字迷宫实验、悬尾实验、强迫游泳实验和避暗实验等方法,观察不同时段的束缚对雌雄ICR小鼠情绪和认知行为的影响。结果将雌雄小鼠分组统计时,在悬尾实验中,雄性白天束缚组的不动时间与雄性空白组相比呈显著性增加(P<0.05)。在强迫游泳实验中,雄性白天束缚组和夜间束缚组的不动时间与雄性空白组相比均显著性增加(P<0.05)。在新奇抑制摄食实验中,与雌性空白组相比,雌性白天束缚组的摄食潜伏期无明显差异,夜间束缚组显著性延长(P<0.05),且夜间与白天束缚组相比呈显著性延长(P<0.05);在夜间束缚时雌性的摄食潜伏期与雄性相比呈显著性延长(P<0.05)。旷场实验中,雌性空白组与雄性空白组相比,中央区时间、中央区/边缘区时间呈显著性降低(P<0.05);雌性白天束缚组的中央区时间、中央区/边缘区时间与雌性空白组相比呈显著性降低(P<0.05)。在高架十字迷宫实验和Y迷宫实验中,各组均无明显差异。避暗实验中,雄性白天束缚组的入暗潜伏期与雄性空白组相比无明显差异,夜间束缚组的入暗潜伏期与空白组相比显著性缩短(P<0.05)。将雌雄小鼠合并统计时,在悬尾实验中,白天雌雄束缚组的不动时间呈显著性增加(P<0.05);在强迫游泳实验中,白天和夜间雌雄束缚组的不动时间呈显著性增加(P<0.01,P<0.05);在旷场实验中,白天雌雄束缚组的中央Objective To investigate the effects of chronic restraint stress for 28 days(day and night)on mood and cognitive-like behavior in male and female ICR mice,to provide a basis for the selection of sex and restraint period in chronic restraint stress model animals.MethodsA total of 72 male and female(1∶1)ICR mice were divided into six groups:male control,daytime restraint,and nighttime restraint groups,and female control,daytime restraint,and nighttime restraint groups.Mice in all but the control groups were bound for 10 h/d and restrained continuously for 28 days to establish a chronic restraint stress model.The emotional and cognitive behaviors induced by restraint in male and female mice at different times were observed by open field,Y maze,novel inhibition feeding,elevated cross maze,tail suspension,forced swimming,and dark-avoidance experiments.ResultsIn the tail suspension experiment,the immobility time of male mice in the daytime restraint group was significantly increased compared with that in the control group(P<0.05),and the immobility times of male mice in the daytime and nighttime restraint groups were also significantly increased in the forced swimming experiment,compared with those in the control group(P<0.05).There was no significant difference between the female daytime restraint and female control groups in the novelty inhibition feeding experiment,but the feeding latency of the nighttime restraint group was significantly longer than that of the control group(P<0.05)and the daytime restraint group(P<0.05).The feeding latency of female mice was significantly longer than that of males during nighttime restraint(P<0.05).In the open field test,compared with the male control group,the female control group showed a significant decrease in central area time and the ratio of central area time to peripheral area time(P<0.05).Compared with the female control group,the female daytime restraint group exhibited a significant decrease in central area time and the ratio of central area time to peripheral area ti

关 键 词：慢性束缚应激模型 抑郁症 焦虑症 认知障碍 

分 类 号：R-33[医药卫生]