痘苗病毒VR-1354感染C57BL/6N和BALB/C小鼠模型的构建及差异比较研究  

Construction and comparative study of vaccinia virus VR-1354 infection models in C57BL/6N and BALB/C mice

作　　者：吴云祥 王超[2] 李顺[2] 周晓辉[2] WU Yunxiang;WANG Chao;LI Shun;ZHOU Xiaohui(Shanghai Normal University,College of Life Sciences,Shanghai 200233,China;Laboratory Animal Department,Shanghai Public Health Clinical Center,Shanghai 201508)

机构地区：[1]上海师范大学生命科学学院,上海200233 [2]上海市公共卫生临床中心实验动物部,上海201508

出　　处：《中国比较医学杂志》2025年第2期94-100,共7页Chinese Journal of Comparative Medicine

基　　金：国家重点研发计划基础科研条件与重大科学仪器设备研发专项(2023YFF0724800,2023YFF0724801)。

摘　　要：目的构建痘苗病毒VR-1354(WR(NIH TC-adapted))感染C57BL/6N和BALB/C小鼠的模型,并比较C57BL/6N小鼠与BALB/C小鼠感染模型的差异。方法按照常规方法,扩增痘苗病毒VR-1354并进行浓缩和滴定。分别测定痘苗病毒VR-1354对C57BL/6N小鼠和BALB/C小鼠的半数致死量(LD50)。使用LD50分别感染两种品系小鼠,取对照组及实验组小鼠肺组织,于第3、7和14天进行苏木素-伊红(HE)染色,同时测量肺组织病毒载量变化。结果扩增后的痘苗病毒VR-1354的滴度为3×10^(8)PFU/mL。痘苗病毒VR-1354感染C57BL/6N小鼠的LD50为2.5×10^(3)PFU,感染BALB/C小鼠的LD_(50)为2.8×10^(3)PFU。使用2.5×10^(3)PFU痘病毒VR-1354感染两种品系小鼠后,随着感染时间增加小鼠肺组织病变更严重,但第7天后小鼠症状趋于恢复。两种品系小鼠的肺组织病毒载量在第3天时开始增加,在第7天达到最大值,之后逐渐降低。结论成功建立痘苗病毒VR-1354感染C57BL/6N小鼠和BALB/C小鼠的感染模型,该病毒可以引起小鼠肺组织的明显病变,包括炎性细胞浸润和肺泡腔液体渗出等。两种品系的小鼠感染模型在发病程度以及易感性方面存在一定差异,C57BL/6N小鼠对于该病毒的易感性高于BALB/C小鼠。Objective To construct mouse models of vaccinia virus infection using C57BL/6N and BALB/C mice infected with vaccinia virus VR-1354(WR(NIH TC-adapted)),and to compare the differences between the models in the two mouse strains.MethodsVaccinia virus VR-1354 was amplified,concentrated,and titrated,according to conventional method.The median lethal doses(LD50)of vaccinia virus VR-1354 for C57BL/6N and BALB/C mice were determined,respectively,and mice from the two strains were infected with the respective LD50.Lung tissues from control and experimental mice were removed for hematoxylin and eosin staining on days 3,7,and 14,and changes in the virus load in the lung tissues were measured at the same times.ResultsThe titer of the amplified vaccinia virus VR-1354 was 3×10^(8)plaque-forming units(PFU)/mL.The LD_(50) values in C57BL/6N and BALB/C mice were 2.5×10^(3)and BALB/C 2.8×10^(3)PFU,respectively.After infecting mice from both strains with 2.5×10^(3)PFU of vaccinia virus VR 1354,lung tissue lesions worsened with increasing infection time,but the symptoms of the mice tended to recover after day 7.The virus loads in lung tissues began to increase on day 3 in both strains,peaked on day 7,and then gradually decreased.ConclusionsC57BL/6N and BALB/C mouse models infected with vaccinia virus VR-1354 were established successfully.The virus can cause obvious lesions in mouse lung tissues,including inflammatory cell infiltration and alveolar cavity fluid exudation.The degree of disease onset and susceptibility differed between the two mouse strains,with C57BL/6N mice showing higher susceptibility to this virus than BALB/C mice.

关 键 词：C57BL/6N BALB/C VR-1354 感染模型 比较研究 

分 类 号：R-33[医药卫生]